Mechanism of intracellular persistence of Staphylococcus aureus by novel function of toxic shock syndrome toxin-1

中毒性休克综合征毒素-1的新功能对金黄色葡萄球菌细胞内持久性的机制

• 批准号: 23790467
• 负责人: ASANO Krisana
• 金额: $ 2.75万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790467/
• 关键词: 黄色ブドウ球菌; TSST-1; オートファジー; 感染症; 細胞内寄生

Effect of toxic shock syndrome toxin-1 (TSST-1) on Staphylococcus aureus infection in the epithelial cells was investigated. TSST-1 did not alter the adhesion and invasion into the epithelial cells, HeLa 229, HEK293 and 407. However, TSST-1 reduced the accumulation of autophagosomes in nutrient starved cells or rapamycin-treated cells, suggesting that TSST-1 suppresses autophagy. In addition, this suppression did not require superantigenic activity and might be involved in the autophagosome formation process rather than autophagosome degradation. From day 5 after infection, intracellular bacterial number and cytotoxicity of TSST-1-producing S. aureus were deceased in comparison to non-producing strain, suggesting that TSST-1 may promote intracellular persistence of S. aureus in the epithelial cells.

研究了中毒性休克综合征毒素-1（TSST-1）对金黄色葡萄球菌感染上皮细胞的影响。TSST-1不改变对上皮细胞HeLa 229、HEK 293和407的粘附和侵袭。然而，TSST-1减少了自噬体在营养饥饿细胞或雷帕霉素处理的细胞中的积累，表明TSST-1抑制自噬。此外，这种抑制不需要超抗原活性，可能参与自噬体形成过程，而不是自噬体降解。从感染后第5天开始，产TSST-1的S.结果表明，TSST-1能促进金黄色葡萄球菌的胞内持久性。金黄色葡萄球菌在上皮细胞。

项目成果

Alteration of intestinal microbiota in mice orally administered with salmon cartilage proteoglycan, a prophylactic agent.

• DOI: 10.1371/journal.pone.0075008
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Asano K;Yoshimura S;Nakane A
• 通讯作者: Nakane A

Autophagy suppression by Toxic Shock Syndrome Toxin-1 in superantigenicacitivity-independent manner

中毒性休克综合征 Toxin-1 以不依赖超抗原活性的方式抑制自噬

• 发表时间: 2014
• 作者: 渡邊真弥;祝弘樹;船渡川圭次;はい島由二;奥村香世;加藤雅子;橋本雅仁;切替富美子;秋山徹;切替照雄;Asano K and Nakane A
• 通讯作者: Asano K and Nakane A

Superantigenic-independent activity of Toxic Shock Syndrome Toxin-1 in suppression of autophagy

中毒性休克综合征 Toxin-1 抑制自噬的超抗原依赖性活性

• 发表时间: 2013
• 作者: 祝弘樹;船渡川圭次;渡邊真弥;奥村香世;はい島由二;加藤雅子;切替富美子;秋山徹;切替照雄;Asano K and Nakane A
• 通讯作者: Asano K and Nakane A

Inhibition of emetic and superantigenic activities of staphylococcal enterotoxin A by synthetic peptides

• DOI: 10.1016/j.peptides.2012.08.013
• 发表时间: 2012-11-01
• 期刊: PEPTIDES
• 影响因子: 3
• 作者: Maina, Edward K.;Hu, Dong-Liang;Nakane, Akio
• 通讯作者: Nakane, Akio

Autophagic suppressing activity of staphylococcal Toxic Shock Syndrome Toxin-1

葡萄球菌中毒性休克综合征Toxin-1的自噬抑制活性

• 发表时间: 2013
• 作者: Shin-Ichi Inoue;Mamoru Niikura;Megumi Inoue;Fumie Kobayash;Asano K. and Nakane A
• 通讯作者: Asano K. and Nakane A