HIV-1 Rev protein remodels viral export RNP

HIV-1 Rev蛋白重塑病毒输出RNP

• 批准号: 23790501
• 负责人: TANIGUCHI Ichiro
• 金额: $ 2.5万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790501/
• 关键词: RNA 核外輸送; HIV-1; Revタンパク質; RNA核外輸送; RNP; スプライシング

Human immunodeficiency virus type 1 (HIV-1) Rev protein induces export of the singly spliced and the unspliced HIV-1 transcripts. However, how these RNAs are exported is not well understood, since such transcripts should have both the transcription/export (TREX)complex and Rev on the same RNA, and therefore should have possibility of utilizing the TAP-dependent or CRM1-dependent export, or both. When HIV-1 transcripts were expressed in HEK293T cells, it was suggested that Rev suppresses the TAP recruitment to those transcripts. We also found that Rev interacts with the cap-binding complex (CBC) and inhibits the recruitment of Aly/REF, an adaptor for the TAP-dependent export.

人类免疫缺陷病毒1型（HIV-1） Rev蛋白诱导单剪接和非剪接HIV-1转录本的输出。然而，这些RNA是如何输出的还不是很清楚，因为这些转录本应该在同一RNA上同时具有转录/输出（TREX）复合体和Rev，因此应该有可能利用tap依赖或crm1依赖的输出，或两者兼有。当HIV-1转录本在HEK293T细胞中表达时，表明Rev抑制TAP对这些转录本的募集。我们还发现Rev与帽结合复合体（CBC）相互作用并抑制Aly/REF的募集，Aly/REF是tap依赖性输出的适配器。

项目成果

A Novel Activity of HIV-1 Rev Protein to Remodel Export RNPs in DirectingVirus-specific RNA Export

HIV-1 Rev 蛋白重塑导出 RNP 从而指导病毒特异性 RNA 导出的新活性

• 发表时间: 2011
• 作者: Ichiro Taniguchi;Naoto Mabuchi;Mutsuhito Ohno
• 通讯作者: Mutsuhito Ohno

A Novel Activity of HIV-1 Rev Protein to Remodel Export RNPs in Directing Virus-specific RNA Export

HIV-1 Rev 蛋白重塑导出 RNP 从而指导病毒特异性 RNA 导出的新活性

• 发表时间: 2011
• 作者: Ichiro Taniguchi;Naoto Mabuchi;Mutsuhito Ohno
• 通讯作者: Mutsuhito Ohno

hnRNP C Tetramer Measures RNA Length to Classify RNA Polymerase II Transcripts for Export

• DOI: 10.1126/science.1218469
• 发表时间: 2012-03-30
• 期刊: SCIENCE
• 影响因子: 56.9
• 作者: McCloskey, Asako;Taniguchi, Ichiro;Ohno, Mutsuhito
• 通讯作者: Ohno, Mutsuhito

Mechanistic insights into human pre-mRNA splicing of human ultra-short introns: potential unusual

对人类超短内含子的人类前mRNA剪接的机制见解：潜在的不寻常

• 发表时间: 2012
• 期刊: Biochem Biophys Res Commun.
• 作者: Sasaki-Haraguchi N;Shimada MK,Taniguchi I;Ohno M;Mayeda A.
• 通讯作者: Mayeda A.

Mechanistic insights into human pre-mRNA splicing of human ultra-short introns: Potential unusual mechanism identifies G-rich introns

• DOI: 10.1016/j.bbrc.2012.05.112
• 发表时间: 2012-06-29
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Sasaki-Haraguchi, Noriko;Shimada, Makoto K.;Mayeda, Akila
• 通讯作者: Mayeda, Akila