HIV-1 Rev protein remodels viral export RNP

人类免疫缺陷病毒1型（HIV-1）Rev蛋白诱导单剪接和未剪接HIV-1转录物的输出。然而，这些RNA是如何输出的还没有很好的理解，因为这样的转录本应该在同一RNA上具有转录/输出（TREX）复合物和Rev，因此应该有可能利用TAP依赖性或CRM 1依赖性输出，或两者兼而有之。当HIV-1转录物在HEK 293 T细胞中表达时，表明Rev抑制TAP募集到这些转录物。我们还发现，Rev与帽结合复合物（CBC）相互作用，并抑制招募的Aly/REF，衔接TAP依赖的出口。

Human immunodeficiency virus type 1 (HIV-1) Rev protein induces export of the singly spliced and the unspliced HIV-1 transcripts. However, how these RNAs are exported is not well understood, since such transcripts should have both the transcription/export (TREX)complex and Rev on the same RNA, and therefore should have possibility of utilizing the TAP-dependent or CRM1-dependent export, or both. When HIV-1 transcripts were expressed in HEK293T cells, it was suggested that Rev suppresses the TAP recruitment to those transcripts. We also found that Rev interacts with the cap-binding complex (CBC) and inhibits the recruitment of Aly/REF, an adaptor for the TAP-dependent export.