The role for Laptm5 in the down-modulation of pre-B cell receptor

Laptm5 在前 B 细胞受体下调中的作用

• 批准号: 23790530
• 负责人: KAWANO Youhei
• 金额: $ 2.83万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790530/
• 关键词: プレB細胞; pre-BCR; リソソーム; タンパク分解

Pre-B cell receptor (pre-BCR) expression has to be strictly regulated for normal B cell development, and its dysregulation is associated with anomalies of B-lineage cells, including leukemogenesis. However, it remained elusive on the mechanism underlying pre-BCR down-modulation although transcriptional silencing of pre-BCR component is currently proposed. In this paper, we found that a lysosomal membrane protein, Laptm5 is up-regulated via autonomous pre-BCR signaling, followed by pre-BCR down-modulation by promoting its degradation in lysosomes. Of note, this finding suggests there is another mechanism of pre-BCR down-modulation at the protein level through the regulation of Laptm5.

前B细胞受体(Pre-B cell Receptor，Pre-BCR)的表达必须受到严格的调控才能使B细胞正常发育，其异常与B细胞系的异常有关，包括白血病的发生。然而，尽管目前提出了转录沉默Pre-BCR组分，但对Pre-BCR下调的机制仍不清楚。在本文中，我们发现溶酶体膜蛋白Laptm5通过自主的Pre-BCR信号上调，然后通过促进其在溶酶体中的降解而下调Pre-BCR。值得注意的是，这一发现表明，通过调节Laptm5，在蛋白质水平上存在另一种前BCR下调的机制。

项目成果

A Novel Mechanism for the Autonomous Termination of Pre-B Cell Receptor Expression via Induction of Lysosome-Associated Protein Transmembrane 5

• DOI: 10.1128/mcb.00531-12
• 发表时间: 2012-11-01
• 期刊: MOLECULAR AND CELLULAR BIOLOGY
• 影响因子: 5.3
• 作者: Kawano, Yohei;Ouchida, Rika;Karasuyama, Hajime
• 通讯作者: Karasuyama, Hajime

LAPTM5 down-modulates pre-B cell receptor in an autophagy-independent manner

LAPTM5 以不依赖自噬的方式下调前 B 细胞受体

• 作者: Ito;T.;Kitajima;M.;Tumes;J. D.;Endo;Y.;Onodera;A.;Hashimoto;K.;Motohashi;S.;Yamashita;M.;Nishimura;T.;and Nakayama;T.;河野洋平
• 通讯作者: 河野洋平

A novel mechanism for the autonomous termination of pre-B cell receptor expression via induction of lysosomal-associated protein transmembrane

通过诱导溶酶体相关蛋白跨膜自主终止前 B 细胞受体表达的新机制

• 发表时间: 2013
• 作者: Honda K;Atarashi K;Nishio J.;Yohei Kawano
• 通讯作者: Yohei Kawano

Laptm5 down-modulates pre-B cell receptor in anautophagy-independent manner

Laptm5 以不依赖自噬的方式下调前 B 细胞受体

• 发表时间: 2011
• 作者: Kawano;Y.;Ouchida;R.;O-Wang;J.;Yoshikawa;S.;Yamamoto;M.;Kitamura;D.;Karasuyama;H.
• 通讯作者: H.