Roles of Notch signal regulating microRNA in T cell differentiation

Notch信号调节microRNA在T细胞分化中的作用

• 批准号: 23790538
• 负责人: NISHIDA Jun
• 金额: $ 2.41万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790538/
• 关键词: miR449a; T cell; Notch; miRNA; Tリンパ球

We analyzed expression of micro RNA in Notch1 intercellular domain over expressed DO11.10 cells. We identified one of micro RNA, miR449a, which expression was controlled under the Notch signaling. To clarified role of miR449a, we established miR449a knock out mouse. We compared development and maturation of immune competent cell between wild type and miR449a KO mouse. However, we could not find any difference on differentiation of immune competent cell at thymus, spleen and other secondary lymphoid tissue. We also analyzed immune competent cell in small intestinal lamina propria and intraepithelial lymphocytes, we could not find any difference between wild and miR449a KO mouse.

我们分析了在表达的DO11.10细胞中Notch1胞间结构域的微小RNA的表达。我们鉴定了一个微小的RNA，miR449a，它的表达受Notch信号的调控。为了阐明miR449a的作用，我们建立了miR449a基因敲除小鼠。我们比较了野生型和miR449A KO小鼠免疫活性细胞的发育和成熟。但在胸腺、脾等次级淋巴组织中，未发现免疫活性细胞分化的差异。我们还分析了小肠固有层和上皮内淋巴细胞的免疫活性细胞，我们没有发现野生小鼠和miR449A KO小鼠有任何差异。