Analysis of the mechanisms of allopurinol-induced severe cutaneous adverse reactions

为了研究别嘌呤醇或苯妥英是否产生药物-蛋白加合物，我们将这两种药物分别与不同的人肝组织孵育。因此，细胞色素P450活性和谷胱甘肽浓度对苯妥英药物-蛋白加合物的生成具有重要意义。此外，虽然没有报道P450活性参与别嘌呤醇代谢，但本研究结果表明P450活性在药物-蛋白加合物的生成中发挥了作用。

To investigate whether allopurinol or phenytoin generate drug-protein adduct, each drugs were incubated with various human liver tissues. As a result, it is suggested that cytochrome P450 activities and the concentration of glutathione in cells are of importance for generation of drug-protein adduct formation with phenytoin. In addition, although it have not been reported that P450 activities are involved in the allopurinol metabolism, the result of this study suggested that P450 activities played a role in generation of the drug-protein adduct.