The analysis of novel neuropathic pain related protein in the mechanism of chronic pain.

为了阐明神经性疼痛相关蛋白a，-B （NPRP-A, NPRP-B）的作用和功能，我们利用特异性抗体和敲除小鼠分析了NPRP-B的生物学特性和在SNI后的疼痛反应。NPRP-B在包括脊髓背角在内的中枢神经系统中高表达，尤其在突触体部分富集。NPRP-B以磷酸化蛋白形式存在。在NPRP-B敲除小鼠中，与野生型小鼠相比，sni后机械异常性疼痛明显减少。

To clarify the role and functions of Neuropathic Pain RelatedProtein-A,-B (NPRP-A、NPRP-B) , we analyzed biological properties and pain responses after spared nerve injury (SNI) of NPRP-B using specific antibody and knock-out mice. NPRP-B is highly expressed in the central nervous system including spinal dorsal horn and especially enriched in synaptosomal fraction. NPRP-B exists as phosphorylated protein. In NPRP-B knock-out mice, mechanical allodynia is significantly reduced after SNIcompared with that in wild-type mice.