Development of simultaneous micro analysis method for muscle relaxants in biological samples by UPLC-MS/MS

UPLC-MS/MS 开发生物样品中肌肉松弛剂同步微量分析方法

• 批准号: 23790732
• 负责人: OGAWA Tadashi
• 金额: $ 2.83万
• 依托单位: Aichi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790732/
• 关键词: 中枢性筋弛緩薬; 末梢性筋弛緩薬; UPLC-MS/MS; 法中毒; 筋弛緩薬; UPLC-MS-MS; ESI-APCI同時イオン化モード; 筋弛緩剤

In this study, rapid and sensitive methods for simultaneous analysis of muscle relaxants by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) were developed.Simultaneous analysis of central-acting muscle relaxants by UPLC-MS/MS has been realized by the capability of our instrument for simultaneous selected reaction monitoring of the target compounds in both positive and negative ionization modes. On the other hand, quantification of peripheral non-depolarizing muscle relaxants was difficult by LC-MS/MS with ESI (Electrospray ionization) mode. However, the developed simultaneous analysis method of non-depolarizing muscle relaxants in whole blood by UPLC-MS/MS with ESI-APCI (Atmospheric pressure chemical ionization) mix ionization mode in this study showed better quantitativity.

本研究建立了快速、灵敏的超高效液相色谱-串联质谱（UPLC-MS/MS）同时分析肌松药的方法，利用该仪器在正负离子模式下同时监测目标化合物的选择性反应，实现了中枢作用肌松药的UPLC-MS/MS同时分析。另一方面，外周非去极化肌松药的定量是困难的，通过LC-MS/MS与ESI（电喷雾电离）模式。而本研究建立的全血中非去极化肌松药的超高效液相色谱-串联质谱（UPLC-MS/MS）ESI-APCI（Atmospheric Pressure Chemical Ionization）混合离子化模式同时分析方法具有更好的定量性。

项目成果

UPLC-MS/MSを用いた血漿中筋弛緩剤の一斉分析

使用 UPLC-MS/MS 同时分析血浆中的肌肉松弛剂

• 发表时间: 2011
• 作者: Tadashi Ogawa;Kenjiro Ito;Hideki Hattori;Jun Ueyama;Masae Iwai;Kenya Iwakura;Akira Ishii;Osamu Suzuki;Hiroshi Seno;小川匡之,服部秀樹,金子理奈,伊藤健次郎,岩井雅枝,星野伸忠,有信哲哉,石井晃,鈴木修,妹尾洋
• 通讯作者: 小川匡之,服部秀樹,金子理奈,伊藤健次郎,岩井雅枝,星野伸忠,有信哲哉,石井晃,鈴木修,妹尾洋

Simultaneous analysis of peripheral muscle relaxants in whole blood by ultra-performance liquid chromatography tandem mass spectrometry

超高效液相色谱串联质谱法同时分析全血中的外周肌松弛剂

• 发表时间: 2012
• 作者: Fukudo S;Machida T;Endo Y;Shoji T;Kano M;Kanazawa M.;Tadashi Ogawa
• 通讯作者: Tadashi Ogawa

SIMPLE AND RAPID QUANTITATION OF 21 BILE ACIDS IN RAT SERUM AND LIVER BY UPLC-MS-MS: EFFECT OF HIGH FAT DIET ON GLYCINE CONJUGATES OF RAT BILE ACIDS

• DOI: 10.18999/nagjms.75.1-2.57
• 发表时间: 2013-02
• 期刊: Nagoya Journal of Medical Science
• 影响因子: 0.9
• 作者: Yudai Suzuki;R. Kaneko;Mina Nomura;Hisao Naito;K. Kitamori;T. Nakajima;Tadashi Ogawa;H. Hattori;H. Seno;A. Ishii

High-throughput and simultaneous analysis of eight central-acting muscle relaxants in human plasma by ultra-performance liquid chromatography-tandem mass spectrometry in the positive and negative ionization modes

超高效液相色谱-串联质谱法在正负电离模式下高通量同时分析人血浆中的八种中枢作用肌松剂

• 发表时间: 2011
• 期刊: Analytical and Bioanalytical Chemistry
• 影响因子: 4.3
• 作者: Tadashi Ogawa;Hideki Hattori;Rina Kaneko;Kenjiro Ito;Masae Iwai;Yoko Mizutani;Tetsuya Arinobu;Akira Ishii;Hiroshi Seno
• 通讯作者: Hiroshi Seno

Simultaneous and sensitive analysis of fourth-generation antidepressants in human plasma by ultra-performance liquid chromatography-tandem mass spectrometry

超高效液相色谱-串联质谱法同时灵敏分析人血浆中的第四代抗抑郁药

• DOI: 10.1007/s11419-013-0197-2
• 发表时间: 2014
• 期刊: Forensic Toxicology
• 影响因子: 2.2
• 作者: Iwakura K;Ogawa T;Hattori H;Iwai M;Suzuki O;Nakagawa T
• 通讯作者: Nakagawa T