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Structural analyses of IgE-binding proteins originated from plant foodstuffs

植物性食品中 IgE 结合蛋白的结构分析

基本信息

批准号：
09460063
负责人：
OGAWA Tadashi
金额：
$ 4.48万
依托单位：
KYOTO UNIVERSITY (1998)The University of Tokushima (1997)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

Patients allergic to plant foodstuffs are known to increase yearly. We have investigated the allergenic proteins in plant foodstuffs using patient's sera and revealed that patient's IgE are cross-reactive among many homlogous proteins which are widely distributed in paint kingdom. Some of the IgE-binding proteins derived from different foodstuffs are glycoproteins and react with antibody raised against horse radish peroxidase (anti HRP). This antiHRP is known to recognize a asparagine-conjugated and high mannose type sugar moiety on peroxidase. The binding activity of patient's IgE against plant's allergens was reduced remarkedly by the pre-treatment of allergenic proteins with antiHRP, suggesting that the epitopes of these allergens are homologous and to be high mannose type sugar moieties. If these sugar moieties are common epitopes of plant allergens, patient's IgE antibodies could react with many homologous proteins which have similar sugar moiety in plant foodstuffs and patients present allergic symptons against many plants beyond there classification, such as species, genus, and families. We investigate one of the plant allergen which has asparagine-conjugated high mannose type sugar moiety, Gly m Bd 28K and potato's allergen, patatin. Both the allergenic proteins found to have a common epitope against patient's IgE antibodies. Furthermore, we revealed that many other plant proteins might share the common epitopes against patient's sera.

对植物性食物过敏的病人每年都在增加。我们用病人血清研究了植物性食品中的过敏蛋白，发现病人的IgE与油漆王国中广泛分布的许多同源蛋白有交叉反应。一些来自不同食物的IgE结合蛋白是糖蛋白，与辣根过氧化物酶（抗HRP）抗体反应。已知该抗HRP识别过氧化物酶上的天冬酰胺缀合的高甘露糖型糖部分。用抗HRP预处理过敏原蛋白，可显著降低患者IgE对植物过敏原的结合活性，提示这些过敏原的表位是同源的，且为高甘露糖型糖基。如果这些糖部分是植物过敏原的共同表位，患者的IgE抗体可以与植物食品中具有相似糖部分的许多同源蛋白质反应，并且患者对许多超出其分类的植物（例如种、属和科）呈现过敏症状。本文对一种含有天冬酰胺结合的高甘露糖型糖基的植物过敏原Gly m Bd 28K和马铃薯过敏原patatin进行了研究。发现这两种过敏原蛋白具有针对患者IgE抗体的共同表位。此外，我们发现许多其他植物蛋白可能具有针对患者血清的共同表位。