13C-butyrate breath-test is a new non-invasive method for assessing colitis in murine model

在这项研究中，我们首次表明，13 C-丁酸呼气试验与通常用于评估葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎的炎症阶段的参数有很好的相关性。由于在三硝基苯磺酸（TNBS）诱导的小鼠结肠炎中13 CO2的排泄显著低于正常小鼠，我们证实了可以通过13 C-丁酸呼气试验来评估各种类型的小鼠结肠炎。此外，我们已经证明了汉方药物给药后13 CO2排泄的恢复。我们的数据表明，13 C-丁酸呼气试验可能反映汉方药物治疗DSS-小鼠结肠炎的疗效。总之，13 C-丁酸呼气试验是一种新建立的非侵入性方法，用于评估小鼠结肠炎。它可以提供结肠炎症的实时信息，因此它可能是实验性结肠炎研究的有用工具。

In this study, we showed for the first time that 13C-butyrate breath-test is well correlated with parameters commonly used for assessing inflammatory phase of Dextran sodium sulphate (DSS)-induced murine colitis. Because excretion of 13CO2in trinitoro benzene sulfonic acid (TNBS)-induced murine colitis was significantly lower than those of the normal mice, we confirmed that various types of murine colitis could be assessed by 13C-butyrate breath-test. Moreover, we have demonstrated the recovery of 13CO2excretion after the administration of Kampo medicine. Our data suggest that 13C-butyrate breath-test might reflect therapeutic efficacy of Kampo medicine in DSS-murine colitis. In conclusion, 13C-butyrate breath-test is newly established, non-invasive method for assessing murine colitis. It may provide real-time information on colonic inflammation, so it may be a useful tool in research on experimental colitis.