Identification of the apoE for aggravation factor in cardiomyopathy

心肌病加重因素apoE的鉴定

基本信息

批准号：
23790860
负责人：
ARAI Shinobu
金额：
$ 2.66万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2013
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790860/
关键词：
心筋症遺伝子発現解析 apoE 抗酸化作用心不全

项目摘要

Cardiovascular disease is the number 2 cause of death, according to cancer in JAPAN. Cardiac failure has a poor prognosis as ending point of various cardiac disease. The cardiac disease severity in each patientsshow remarkable differences, however the causes leaded to sever still unclear. Transgenic mouse techniques have made significant progress for cardiomyopathy, somany cardiac failure model mice were established. On the other hand, these candidate genes could not be the definitive etiology in practical clinical of cardiac cardiomyopathy. To identify the definitive etiology of sever cardiomyopathy, we analyzed the DNA microarray analysis of cardiac muscle obtained from cardiac failure patients. In this study, to reveal the distinct factor of severity, 36 subjectes with differentseverity grades of cardiac failure were elected (myocardial stress marker BNP 11-8091 pg/ml, left ventricular ejection fraction LVEF 12-79%). In this results, 17 statistically significant increased genes (p<0.01,ratio > 1.8) were detected in patients decreased LVEF and developed left ventricular dilatation. 17 genes include BNP and fibrosisgenes (CTGF, collagen genes and so on). On the other hand, 13 statistically significant increased genes (p<0.01, ratio < -1.8) were detected. These genes have not been focused on relation with cardiac disease until now, however, these shown an excellent correlation with LVEF. We will reports the DNA microarray results of cardiomyopaty patients and the possibility of newly factor as cardiac severity.

根据日本癌症的数据，心血管疾病是死亡的第二名。作为各种心脏病的终点，心脏衰竭的预后较差。每位患者的心脏病严重程度显着差异，但是导致断裂的原因仍然不清楚。转基因小鼠技术已经在心肌病，SOMINIC心脏衰竭模型小鼠方面取得了重大进展。另一方面，这些候选基因不可能是心脏心肌病的实践临床的确定性病因。为了确定严重心肌病的确定病因，我们分析了从心力衰竭患者获得的心脏肌肉的DNA微阵列分析。在这项研究中，为了揭示严重程度的不同因素，选出了36名具有不同性心脏衰竭等级的受试者（心肌应力标记物BNP BNP 11-8091 pg/ml，左心室射血分数LVEF 12-79％）。在此结果中，在患者中检测到17个具有统计学意义的基因（p <0.01，比率> 1.8），降低了LVEF并发展出左心室扩张。 17个基因包括BNP和纤维化基因（CTGF，胶原基因等）。另一方面，检测到13个具有统计学意义的基因（p <0.01，比率<-1.8）。到目前为止，这些基因尚未集中在与心脏病的关系上，但是这些基因与LVEF有着良好的相关性。我们将报告心肌患者的DNA微阵列结果，以及新近因素作为心脏严重程度的可能性。