The development of the mechanism of inflammation regulation and the biomarker in IgA nephropathy with the use of glycomics

利用糖组学研究IgA肾病炎症调节机制和生物标志物

• 批准号: 23790943
• 负责人: INOUE Tatsuyuki
• 金额: $ 2.75万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790943/
• 关键词: 腎臓学; IgA 腎症; IgA腎症; 糖鎖解析; アディポネクチン; バイオマーカー

We analyzed the mechanism of glomerular inflammation by the aberrantly glycosylated IgA. The expression of adiponectin was downregulated in cultured human glomerular cellsstimulated by the deglycosylated IgA, and was also in glomeruli in the patients of IgA nephropathy.We developed the methods of the aberrantly glycosylated IgA in patients with IgA nephropathy . The deglycosylated IgA in the renal tissue was certified by the staining of HA lectin. The urine deglycosylated IgA I was certified by the methods combined with two lectins, the urine deglycosylated IgA in the patients with IgA nephropathy was significantly increased compared with the other kidney disease.

我们通过IgA糖基化伊加来分析肾小球炎症的机制。在体外培养的人肾小球细胞中，去糖基化伊加刺激后脂联素表达下调，伊加肾病患者肾小球中脂联素表达也下调，我们建立了伊加肾病患者异常糖基化伊加的检测方法。HA凝集素染色证实肾组织中存在去糖基化伊加。两种凝集素联合检测尿去糖基伊加I，伊加肾病患者尿去糖基伊加明显高于其他肾脏疾病患者。

项目成果

Suppression of adiponectin by aberrantly glycosylated IgA1 in glomerular mesangial cells in vitro and in vivo.

• DOI: 10.1371/journal.pone.0033965
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Inoue T;Sugiyama H;Kitagawa M;Takiue K;Morinaga H;Ogawa A;Kikumoto Y;Kitamura S;Maeshima Y;Makino H
• 通讯作者: Makino H

Aberrantly glycosylated IgA1 diminishes adiponectin expression in glomerular mesangial cells in vitro and in vivo: A novel anti-inflammatory mechanism in IgA nephropathy

异常糖基化 IgA1 减少体外和体内肾小球系膜细胞脂联素表达：IgA 肾病的新型抗炎机制

• 发表时间: 2011
• 作者: Hitoshi Sugiyama;Tatsuyuki Inoue;Masashi Kitagawa;Keiichi Takiue;Hiroshi Morinaga;Yoko Kikumoto;Ayu Ogawa;Shinji Kitamura;Yohei Maeshima;Hirofumi Makino
• 通讯作者: Hirofumi Makino

腎生検組織における HA レクチン染色はIgA 腎症の新規診断法として有用である

肾活检组织中的 HA 凝集素染色可作为 IgA 肾病的新诊断方法

• 发表时间: 2011
• 作者: 井上達之;杉山 斉;北川正史;瀧上慶一;森永裕士;喜多村真治;前島洋平;槇野博史
• 通讯作者: 槇野博史