Elucidation of the inhibitor with the protein to induce endocytosis of podocin in podocytes

阐明诱导足细胞内吞足蛋白的蛋白抑制剂

• 批准号: 23790956
• 负责人: HIDAKA Teruo
• 金额: $ 2.75万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790956/
• 关键词: podocin; SNX9; synaptopodin; glomerulonephritis; irreversible change; 足細胞; スリット膜; sorting nexin 9; エンドサイトーシス; 糸球体腎炎; ポドシン; ポドサイト

We noticed that the deference pattern of staining with podocin and synaptopodin, which are slit-diaphragm proteins in irreversible glomerulonephritis. We found that Sorting Nexin 9 (SNX9) lead the podocin trafficking in these nephritis. Actually, we recognized that SNX9 will appear in glomeruli only significant severe glomerulonephritis, and it connect tightly to podocin in such conditions. In biochemical examination also indicated SNX9 and podocin contact each other, and the immunofluorescence examination showed same staining pattern in irreversible glomerulonephritis. We are now continuing to research of SNX9 and podocin because of completion of our hypothesis with knocked down and over expression of SNX9 podocyte cell line.

我们注意到不可逆性肾小球肾炎中的裂膜蛋白podocin和synaptopodin的染色模式不同。我们发现，排序连接蛋白9（SNX9）导致podocin运输在这些肾炎。实际上，我们认识到SNX9只会出现在肾小球中显著严重的肾小球肾炎，并且在这种情况下它与podocin紧密连接。在生化检查中也显示SNX9和podocin相互接触，免疫荧光检查在不可逆性肾小球肾炎中显示相同的染色模式。我们现在正在继续研究SNX9和podocin，因为我们的假设已经完成，敲低和过量表达SNX9足细胞系。

项目成果

Dendrin location in podocytes is associated with the disease progression in animal and human glomerulopathy

足细胞中树突蛋白的位置与动物和人类肾小球病的疾病进展相关

• 发表时间: 2011
• 期刊: Am J Nephrol
• 影响因子: 4.2
• 作者: Asanuma K;Akiba-Takagi M;Kodama F;Asao R;Nagai Y;Lydia A;Fukuda H;Tanaka E;Shibata T;Takahara H;Hidaka T;Asanuma E;Kominami E;Ueno T;Tomino Y
• 通讯作者: Tomino Y