Elucidation of the inhibitor with the protein to induce endocytosis of podocin in podocytes

我们注意到不可逆肾小球肾炎中裂孔-隔膜蛋白podocin和synaptopodin染色的差异。我们发现排序连接蛋白9 （SNX9）在这些肾炎中主导足docin的转运。实际上，我们认识到SNX9只会出现在严重的肾小球肾炎中，并且在这种情况下它与podocin紧密连接。生化检查也显示SNX9与podocin相互接触，免疫荧光检查在不可逆性肾小球肾炎中显示相同的染色模式。我们现在正在继续研究SNX9和podocin，因为我们的假设完成了SNX9足细胞的敲除和过表达。

We noticed that the deference pattern of staining with podocin and synaptopodin, which are slit-diaphragm proteins in irreversible glomerulonephritis. We found that Sorting Nexin 9 (SNX9) lead the podocin trafficking in these nephritis. Actually, we recognized that SNX9 will appear in glomeruli only significant severe glomerulonephritis, and it connect tightly to podocin in such conditions. In biochemical examination also indicated SNX9 and podocin contact each other, and the immunofluorescence examination showed same staining pattern in irreversible glomerulonephritis. We are now continuing to research of SNX9 and podocin because of completion of our hypothesis with knocked down and over expression of SNX9 podocyte cell line.