Identification of new autoantibodies in anti-cyclic citrullinated peptide (CCP) antibodies negative rheumatoid arthritis

用AlphaScreen方法筛选8例抗CCP抗体阴性类风湿因子阴性类风湿关节炎(RA)患者的新自身抗体。用AlphaScreen方法在所有血清中检测到两种候选蛋白，用Western印迹和ELISA法检测自身抗体。虽然用免疫印迹法检测到了自身抗体，但用ELISA法检测的阳性率很低。这些抗体可能成为自身抗体阴性类风湿关节炎的新的自身抗体。接下来，我们将检查这些自身抗体的临床用途。

The new autoantibodies were screened by the AlphaScreen methodusing sera from eight antiCCP antibody-negative rheumatoid factor-negative rheumatoid arthritis (RA) patients. In two candidate proteins which were detected in all sera by AlphaScreen method, the autoantibodies were determined by Western blot and ELISA. Although autoantibodies were detected by Western blot, the detection rate was low by ELISA. These antibodies may become the new autoantibody in the autoantibody-negative RA. Next, we are going to examine the clinical utility of these autoantibodies.