A study of Reye-like syndrome associated with emetic toxin of bacillus cereus

本研究旨在通过串联质谱法（TMS-MS）分析蜡样芽胞杆菌（Bacillus cereus，CA）感染患者的脂肪酸代谢，并探讨B致吐毒素催吐素（cereulide）对脂肪酸代谢的影响。蜡状芽孢杆菌，对脂肪酸代谢的体外酰基肉毒碱探针（IVP）测定培养的成纤维细胞。两例B型糖尿病患者的脂酰化代谢特征。蜡样芽胞杆菌感染显示为长链酰基肉毒碱的非特异性升高。IVP试验结果显示，催泪素可抑制脂肪酸代谢，导致与谷氨酸尿症2型相似的酰基肉毒碱代谢。此外，催泪素对脂肪酸氧化的影响呈浓度依赖性，IVP法测定的酰基肉毒碱谱表明催泪素对脂肪酸氧化的毒性作用在50-100 ng/ml浓度范围内实现。

This study was aimed to investigate the effect on the fatty acid metabolism of the patients with Bacillus cereus infection by an acylcarnitine analysis using tandem mass spectrometry, and to investigate effect of cereulide, emetic toxin from B. cereus, on fatty acid metabolism using in vitro acylcarnitine probe (IVP) assay of cultured fibroblasts. The acylcarnitie-profile of two patients with B. cereus infection revealed to be non-specific elevation of long-chain acylcarnitines. The result of IVP assay revealed that cereulide could inhibit fatty acid metabolism profiles fatty acid metabolism, resulting the similar acylcarnitine-profile to glutaric aciduria type 2. In addition, cereulide impaired fatty acid oxidation in concentration -dependent, the profile of acylcarnitines of IVP assay suggested toxic effect of cereulide on fatty acid oxidation was actualized from concentration of 50-100 ng/ml.