Basic study of autologous transplantation of ex vivo expanding macrophages to secondary lymphedema

我们尝试建立术后水肿小鼠模型，但未能成功。可能的原因是：（1）蓝色染料难以显示荧光。（2）行走后水肿无明显减轻。（3）淋巴结的检出困难。我们接下来使用糖尿病伤口模型，这是淋巴功能受损的已建立模型之一。他汀类药物可能是巨噬细胞的刺激因子。他汀类药物通过促进巨噬细胞功能，显著改善糖尿病创面淋巴管生成。

We tried to establish postoperative lymphedema mouse model but it was failed within the period. The possible reasons are (1)difficulty of visualizing lymphatics by blue dye. (2)edema was not significantly observeded because of quaruped walking. (3)difficulty of the detection of lymph nodes. We next used diabetic wound mo del which is one of the established model of impaired lymphatic function. We focused on statin which can be possible stimulator of macrophages. Statin application significantly improve lymphangiogenesis in diabetic wound model via promoting macrophage func tion.