Epigenomic analysis and development of therapeutic target for malignant melanoma

为了鉴定黑色素瘤中具有干细胞特征的亚群，我们通过荧光激活分选分离细胞并将细胞植入NOD/SCID小鼠并进行球体形成测定。当移植到小鼠时，阿基黑素瘤细胞中的ABCB 5阳性亚群和WM 266细胞中的CD 271阳性细胞比CD 271阴性细胞或ABCB 5阴性细胞的致瘤性更高。我们还分析了慢循环，标记保留细胞。标记保留细胞的球体形成率高于其他细胞，并且标记保留细胞显示CD 271和ABCB 5的表达增加。这些结果表明这些黑色素瘤细胞亚群具有干细胞特征。我们认为对这些细胞表观遗传学变化的进一步分析可以用于检测黑色素瘤的新治疗靶点。

To identify subpopulation having stem cell characteristics in melanoma, we isolated cells by fluorescence-activated sorting and implanted cells to NOD/SCID mouse and performed sphere formation assay. ABCB5 positive subpopulation in AKI melanoma cells and CD271 positive cells in WM266 cells were more tumorigenic than CD271 negative cells or ABCB5 negative cells when grafted to mice. We also analyzed slow cycling, label retaining cell. The rate of sphere formation of label retaining cells was higher than the other cells and label retaining cells showed increased expression of CD271 and ABCB5. These result show these subpopulation in melanoma cells have stem cell characteristics. We think additional analysis of epigenetic change of these cells can be used to detect new therapeutic target for melanoma.