Is the Lyn-ACSL3 system involved in induction of cell polarization ?

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To examine the localizations of c-Src and Lyn tyrosine kinases in polarized cells, we generated c-Src- or Lyn-inducible Madin-Darby canine kidney epithelial cell (MDCK II) lines. Using these inducible cell lines, we revealed that Lyn is largely localized to basolateral membranes but c-Src is present on both apical and basolateral membranes. In non-polarized cells, Lyn but not c-Src is accumulated in the Golgi region after biosynthesis in the cytoplasm and is transported to the plasma membrane through Lyn's association with long-chain acyl-CoA synthetase 3 (ACSL3) on Golgi membranes. Therefore, further elucidation of a role for the Lyn-ACSL3 system in cell polarization is needed.