Analysis of non-coding RNA expression in stem and progenitor cells in mouse testes

小鼠睾丸干细胞和祖细胞非编码 RNA 表达分析

• 批准号: 23659099
• 负责人: OHBO Kazuyuki
• 金额: $ 2.41万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659099/
• 关键词: 幹細胞; 精巣; Non-coding RNA; 細胞分化; ゲノム修飾; 生殖細胞; 前駆細胞; 小分子RNA

In mice, we found that the significant progression of heterochromatin formation occurred at the transition from stem to progenitor cells in adult male germ cells. In addition, the gene expression pattern was also significantly changed at the transition. Since it has been reported that non-coding RNA plays an important role in heterochromatin formation, resulting in changing of gene expression profiles, we analyzed and compared the expression profiles of miRNA and long non-coding RNA between stem cells and progenitor cells in adult male germ cells. We found several miRNA and long non-coding RNA that were specifically expressed in each of the cell populations.

在小鼠中，我们发现异染色质形成的显著进展发生在成年雄性生殖细胞中从干细胞向祖细胞的转变。此外，基因表达模式也发生了显着变化的过渡。鉴于非编码RNA在异染色质形成中起重要作用，导致基因表达谱的改变，我们分析比较了成年男性生殖细胞中干细胞和祖细胞中miRNA和长链非编码RNA的表达谱。我们发现了几种在每个细胞群中特异性表达的miRNA和长非编码RNA。

项目成果

Analysis of epigenetic changes associated with spermatogonia stem cell differentiation

精原干细胞分化相关的表观遗传变化分析

• 发表时间: 2012
• 作者: Kitazawa T;Sato T;Nishiyama K;Asai R;Arima Y;Uchijima Y;Kurihara Y;Kurihara H.;K.Ohbo
• 通讯作者: K.Ohbo

Splice variant HE4-V3 expression is associated with favorable prognosis in pulmonary adenocarcinoma

• DOI: 10.1007/s13277-011-0252-8
• 发表时间: 2012-02-01
• 期刊: TUMOR BIOLOGY
• 作者: Tokuishi, Keita;Yamashita, Shin-ichi;Kawahara, Katsunobu
• 通讯作者: Kawahara, Katsunobu

Serum level of HE4 is closely associated with pulmonary adenocarcinoma progression

• DOI: 10.1007/s13277-012-0499-8
• 发表时间: 2012-09
• 期刊: Tumor Biology
• 作者: S. Yamashita;K. Tokuishi;T. Moroga;Satoshi Yamamoto;K. Ohbo;S. Miyahara;Y. Yoshida;J. Yanagisawa;D. Hamatake;M. Hiratsuka;Y. Yoshinaga;T. Shiraishi;A. Iwasaki;K. Kawahara

Analysis of epigenetic features of spermatogonial stem cells.

精原干细胞的表观遗传特征分析。

• 发表时间: 2012
• 作者: 小池正人;内山安男;太田 啓介;Yuichiro Arima;Katarzyna A. Podyma-Inoue;Ohbo K. Shirakawa T. Yaman Devece R. et al.
• 通讯作者: Ohbo K. Shirakawa T. Yaman Devece R. et al.

マウス精子形成過程における幹細胞と前駆細胞をゲノム修飾からとらえる

通过基因组修饰了解小鼠精子发生过程中的干细胞和祖细胞

• 发表时间: 2012
• 作者: 大保和之;白川峰征;神里亮人;中島久仁子;古関明彦
• 通讯作者: 古関明彦