权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Functional analysis of cell adhesion molecule, JAM-4S, specifically expressed in tissue specific stem cells.

细胞粘附分子 JAM-4S 的功能分析，该分子在组织特异性干细胞中特异性表达。

基本信息

批准号：
17591009
负责人：
OHBO Kazuyuki
金额：
$ 1.92万
依托单位：
Yokohama City University (2006)Keio University (2005)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

In this project, we focused on the analysis of molecule(s), especially cell surface molecule(s), which are commonly expressed in several tissue specific stem cells because tissue specific stem cells show quite similar properties. Then, we have cloned one of the cell adhesion molecules, JAM-4, which are expressed in both hematopoietic stem cells and male germ stem cells.The JAM-4 transcript, named JAM-4S, which we have cloned from stem cells is shorter than that cloned from kidney and lung. The transcript encoded an isoform of JAM-4 protein, which only contain one immunoglobulin domain. Therefore, we analyzed the function of JAM-4S protein as well as JAM-4 protein in the stem cells at male reproduction system and hematopoietic system. In testes, JAM-4S is expressed in gonocytes and spermatogonia at neonatal stage. In hematopoietic system, JAM-4S is expressed in both stem cell-and progenitor-populations in bone marrow cells.In order to understand the biological function in vivo, we have generated the JAM-4 deficient mice. Because it is difficult to generate JAM-4S specific knock out mice, we have generated conventional JAM-4 knock out mice. The JAM-4 deficient mice alive at least 2 years so far and do not show obvious abnormality. The testes specimen of JAM-4 deficient mice showed normal development. The hematopoiesis of the JAM-4 deficient mice was also normal. In testes and bone marrow, the family molecules of JAM-4, such as JAM-A and JAM-B are also expressed. Therefore, we are going to generate JAM-B/JAM-4-double knock out mice to analyze the phenotype on stem cell population.

在这个项目中，我们重点分析了分子(S)，特别是细胞表面分子(S)，这些分子在几种组织特异性干细胞中普遍表达，因为组织特异性干细胞具有非常相似的性质。然后，我们克隆了一种细胞黏附分子JAM-4，它在造血干细胞和雄性生殖细胞中都有表达，我们从干细胞中克隆的JAM-4转录本比从肾脏和肺中克隆的转录本更短。该转录本编码了JAM-4蛋白的一个亚型，该蛋白只包含一个免疫球蛋白结构域。因此，我们分析了JAM-4S蛋白以及JAM-4蛋白在干细胞中在男性生殖系统和造血系统中的作用。在睾丸中，JAM-4S在新生儿期的性细胞和精原细胞中表达。在造血系统中，JAM-4S在骨髓干细胞和祖细胞中都有表达，为了了解JAM-4S在体内的生物学功能，我们建立了JAM-4缺陷小鼠。因为很难产生JAM-4S特异性基因敲除小鼠，所以我们产生了传统的JAM-4基因敲除小鼠。到目前为止，JAM-4基因缺陷小鼠至少存活了2年，没有表现出明显的异常。JAM-4基因缺陷小鼠睾丸标本发育正常。JAM-4基因缺陷小鼠的造血功能也是正常的。在睾丸和骨髓中也表达JAM-4家族分子，如JAM-A和JAM-B。因此，我们将建立JAM-B/JAM-4-双基因敲除小鼠来分析干细胞群体的表型。

项目成果

期刊论文数量（12）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Loss of Tie2 receptor compromises embryonic stem cell-derived endothelial but not hematopoietic cell survival.

Tie2 受体的缺失会损害胚胎干细胞来源的内皮细胞的存活，但不会损害造血细胞的存活。

DOI：
发表时间：
2006
期刊：
Blood 107
影响因子：
0
作者：
Hamaguchi I.;Morisada T.;Azuma M.;Murakami K.;Kuramitsu M.;Mizukami T.;Ohbo K.;Yamaguchi K.;Oike Y.;Dumont DJ.;Suda T.
通讯作者：
Suda T.

A CTX family cell adhesion molecule, JAM4, is expressed in stem cell and progenitor cell populations of both male germ cell and hematopoietic cell lineages