Analysis and varification on self-and nonself-recognition mechanism in progenitor like thymic epithelila cell lines

胸腺上皮祖细胞系自我与非自我识别机制的分析与验证

• 批准号: 23659246
• 负责人: KASAI Michiyuki
• 金额: $ 2.33万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659246/
• 关键词: 胸腺上皮細胞株; 胸腺皮質上皮細胞; 胸腺髄質上皮細胞; 胸腺微小環境; MHC複合体; 自己抗原; 自己ペプチド; 胸腺上皮細胞; 胸腺; サイモプロテアソーム; beta5tサブユニット; プロテアソーム; Tリンパ球レパトア形成; 中枢性免疫寛容

The complexes consisting of self-peptides plus MHC molecules on thymic epithelial cells play important roles for intrathymic T dell-development. However, the molecular mechanisms for formation and expression of such complexes are not understood well. Firstly, it was revealed that thymic epithelial cell lines established by monolayer culture hardly expressed FoxN1, one of functional molecules for T cell-development. In turn, upon aggregation culture of an epithelial cell-fraction prepared from fetal thymus, the epithelial cells expressed the almost same FoxN1-expression level as that in freshly isolated epithelial cells. Those epithelial cells as well as the FoxN1-inducible epithelial cell lines hardly had an ability to re-construct thymic microenvironment. In future, I am going to proceed with the two plans, the establishment of thymic epithelial cell lines stably expressing FoxN1 and the investigation for formation and expression mechanisms of the MHC complexes in these cell lines.

胸腺上皮细胞上由自身肽和MHC分子组成的复合物在胸腺内T细胞发育中起重要作用。然而，这些复合物形成和表达的分子机制尚不清楚。首先，发现单层培养的胸腺上皮细胞系几乎不表达FoxN1， FoxN1是T细胞发育的功能分子之一。反过来，对从胎儿胸腺制备的上皮细胞片段进行聚集培养后，上皮细胞表达的foxn1表达水平与新鲜分离的上皮细胞几乎相同。这些上皮细胞以及foxn1诱导的上皮细胞系几乎没有重建胸腺微环境的能力。在未来，我将继续进行两个计划，建立稳定表达FoxN1的胸腺上皮细胞系，研究这些细胞系中MHC复合物的形成和表达机制。

项目成果

A bone substitute with high affinity for vitamin D-binding protein--relationship with niche of osteoclasts.

• DOI: 10.1111/jcmm.12180
• 发表时间: 2014-01
• 期刊: Journal of cellular and molecular medicine
• 影响因子: 5.3
• 作者: Ikeda T;Kasai M;Tatsukawa E;Kamitakahara M;Shibata Y;Yokoi T;Nemoto TK;Ioku K
• 通讯作者: Ioku K

胸腺上皮細胞におけるbeta5サブユニットの発現プロファイル

胸腺上皮细胞中β5亚基的表达谱

• 发表时间: 2011
• 作者: Motomura;Y.;et al;笠井道之
• 通讯作者: 笠井道之

Expression profiles of beta 5 subunits in thymic epithelial cell lines

胸腺上皮细胞系中β5亚基的表达谱

• 发表时间: 2011
• 作者: M.Kasai;H.Udono;and Y.Takahama
• 通讯作者: and Y.Takahama

Development and function of cortical thymic epithelial cells.

• DOI: 10.1007/82_2013_322
• 发表时间: 2014
• 期刊: Current topics in microbiology and immunology
• 作者: K. Takada;I. Ohigashi;M. Kasai;H. Nakase;Y. Takahama

Cell-cell contact maintains beta5t expression in cortical thymic epithelial cells.

细胞与细胞的接触维持皮质胸腺上皮细胞中 β5t 的表达。

• 发表时间: 2012
• 作者: M. Kasai;A. Bat-Erdene;and Y. Takahama
• 通讯作者: and Y. Takahama