Property investigation of circulating tumor cells, primary and metastatic cancer tissues by use of whole genome sequencing and application to laboratory testing

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We investigated primary focus, metastatic focus and circulating tumor cells (CTC) in colorectal cancer (CRC) patients by genomics approach in order to deve lop a novel clinical laboratory testing. CRC cell lines were mixed with peripheral blood from healthy volunteer, and isolated from the mixture. Captured by magnetic cell separation and flow cytometry, the cancer cell lines were collected and confirmed by immunostaining with anti-cytokeratin antibody. The technologies were established, however, CTC could not be isolated from CRC patients in early stage. We implemented iTRAQ (isobaric tags for relative and absolute quantitation) method to develop a novel CRC biomarker, and found LRPPRC (Leucine rich PPR motif containing protein) that was expressed 1.62 fold higher in cancer tissues. Tissue-Microarray and immunohistochemistry revealed that the expression level of LRPPRC was related to cancer differentiation. LRPPRC could be a novel marker for prognosis prediction.