Development of method for comprehensive isolation and purification of viral particles from clinical specimens

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A more effective method to purify viral paricles from clinical specimens is required in metagenomic analysis of pathogens using a high-throughput DNA sequencer. In this study, a novel virion purification method using HPLC or field flow fractionation was evaluated. Even in the preliminary results, field flow fractionation combined with a light scattering detector could isolate the virion fraction from a crude virus solution, such as influenza and Newcastle disease virus. In addition, it was demonstrated that hydroxyapatite chromatography effectively purified viral particles from allantoic fluid from an infected embryonated egg. These results suggested that the above methods could be useful for virion fractionation from crude samples.