Metagenomic research of virus infections in human clinical specimens by high-throughput DNA sequencing in Thailand

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Newly developed next-generation sequencing (NGS) technologies can be used to generate large amounts of unbiased data (gigabases) in a single round of operation. So far, we have conducted a direct metagenomic diagnosis of virus infections in human clinical specimens using NGS in Japan. In this study, we collaborated with Osaka University and the Ministry of Public Health, Thailand, to conduct metagenomic research in order to identify causative agents of 36 clinical infectious cases caused by unknown pathogens in Thailand. Several rounds of high-throughput sequencing identified human pathogenic viruses, such as Picornaviridae, Paramyxoviridae, Coronaviridae, Herpesviridae, and Parvoviridae, in 23 specimens.