Development of a novel method for high-throughput discovery of trans- spliced RNAs in mammalian cells

开发一种在哺乳动物细胞中高通量发现转拼 RNA 的新方法

• 批准号: 23659313
• 负责人: IMAMURA Rie
• 金额: $ 2.41万
• 依托单位: Kurume University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659313/
• 关键词: 遺伝子検査学; トランス・スプライシング; バイオテクノロジー

Wedevelopedanovelmethodforhigh-throughputdiscoveryoftrans-splicedRNAsinmammalian cells. Our method is based on a mate-pair analysis, which enables us to collect information of both ends of DNA fragments. In conventional mate-pair analysis, there must contain circularized DNAs derived from multiple DNAs and those mate-pair fragments can be a cause of false positive trans-spliced genes. To solve this problem we developed a novel method, which allows us to prepare circularized DNA libraries in which each circularized DNA is originated from each single DNA. By this method, we can make a systematic survey of the presence of trans-spliced RNAs in mammalian cells in a highly precise manner, and we could find out several candidate genes in hematopoietic stem cells. This method is useful for discovery of trans-spliced RNAs and will enable us to understand the role of trans-spliced RNAs in mammalian.

我们开发了一种新的方法，用于高通量发现哺乳动物细胞中的反式剪接RNA。我们的方法是基于配对分析，这使我们能够收集信息的两端的DNA片段。在传统的配对分析中，必须含有来自多个DNA的环状DNA，这些配对片段可能是导致反式剪接基因假阳性的原因。为了解决这个问题，我们开发了一种新的方法，它允许我们制备环化DNA文库，其中每个环化DNA都来自每个单个DNA。通过这种方法，我们可以以高精度的方式对哺乳动物细胞中存在的反式剪接RNA进行系统的调查，并且我们可以找到造血干细胞中的几个候选基因。这种方法对于发现反式剪接RNA是有用的，并且将使我们能够理解反式剪接RNA在哺乳动物中的作用。

项目成果

A Case of 8p11 Myeloproliferative Syndrome with BCR-FGFR1 Gene Fusion Presenting with Trilineage Acute Leukemia/Lymphoma, Successfully Treated by Cord Blood Transplantation

• DOI: 10.1159/000341289
• 发表时间: 2012-11
• 期刊: Acta Haematologica
• 影响因子: 2.4
• 作者: Satoshi Morishige;E. Oku;Yuka Takata;Yoshizo Kimura;F. Arakawa;R. Seki;R. Imamura;Koichi Osaki;Michitoshi Hashiguchi;K. Yakushiji;S. Mizuno;K. Yoshimoto;K. Nagafuji;K. Ohshima;T. Okamura

Unrelated cord blood transplantation for patients with adult T-cell leukemia/lymphoma: experience at a single institute

• DOI: 10.1007/s12185-012-1177-8
• 发表时间: 2012-11-01
• 期刊: INTERNATIONAL JOURNAL OF HEMATOLOGY
• 影响因子: 2.1
• 作者: Nakamura, Takayuki;Oku, Eijiro;Okamura, Takashi
• 通讯作者: Okamura, Takashi

Amebic colitis and liver abscess complicated by high serum procalcitonin in acute myeloid leukemia.

急性髓性白血病并发高血清降钙素原的阿米巴结肠炎和肝脓肿。

• 发表时间: 2012
• 期刊: Kansenshogaku Zasshi.
• 作者: Oku E;Nomura K;Nakamura T;Morishige S;Seki R;Imamura R;Hashiguchi M;Osaki K;Mizuno S;Nagafuji K;Okamura T.
• 通讯作者: Okamura T.

Successful treatment of small cell variant anaplastic large cell lymphoma with allogeneic peripheral blood stem cell transplantation, and review of the literature

异基因外周血干细胞移植成功治疗小细胞变异型间变性大细胞淋巴瘤并文献复习

• DOI: 10.1007/s12185-012-1242-3
• 发表时间: 2013
• 期刊: Int J Hematol
• 影响因子: 2.1
• 作者: Imamura R;Mouri F;Oku E;Okamura T
• 通讯作者: Okamura T