Role of fatty acid elongase Elovl6 in the sucrose preference formation

脂肪酸延伸酶Elovl6在蔗糖偏好形成中的作用

• 批准号: 23659464
• 负责人: MATSUZAKA Takashi
• 金额: $ 2.33万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659464/
• 关键词: 脂肪酸; 嗜好性; 糖尿病

In this study, we investigated the effects of Elovl6 deletion in leptin receptor-deficient db/db mice, a model of type 2 diabetes. We analyzed the body weights, serum glucose, insulin and HbA1c levels of db+/m, db/db, and db/db-Elovl6KO mice. We also performed immunohistochemical staining and quantitative RT-PCR analysis of islet.Although the obesity of db/db-Elovl6KO mice was similar to db/db mice, development of hyperglycemia and glucose intolerance was dramatically improved. Histological examination indicated that Elovl6 deletion was associated with increased islet mass in db/db mice, and this correlated with serum hyperinsulinemia and glucose-responsive insulin secretion. The expression of several inflammatory genes such as TNF-α and MCP-1 were reduced, and the expression of genes related to β cell function such as PDX-1 and insulin were increased in islets from db/db-Elovl6KO mice compared with db/dbmice. These results suggest that Elovl6 is an important factor on both beta-cell mass and function in T2D. Elovl6 inhibition can be a novel therapeutic target for T2D.

在这项研究中，我们研究了Elov 16缺失在瘦素受体缺陷型db/db小鼠（2型糖尿病模型）中的作用。我们分析了db+/m、db/db和db/db-Elov 16 KO小鼠的体重、血清葡萄糖、胰岛素和HbAlc水平。db/db-Elov 16 KO小鼠肥胖程度与db/db小鼠相似，但高血糖和糖耐量异常的发生明显改善。组织学检查表明Elov 16缺失与db/db小鼠中增加的胰岛质量相关，并且这与血清高胰岛素血症和葡萄糖响应性胰岛素分泌相关。与db/db小鼠相比，db/db-Elov 16 KO小鼠胰岛中TNF-α和MCP-1等炎症基因表达降低，PDX-1和胰岛素等β细胞功能相关基因表达增加。这些结果表明Elovl 6是T2 D中β细胞质量和功能的重要因素。Elovl 6抑制可以是T2 D的新的治疗靶标。

项目成果

Deletion of Elovl6 protects against thefailure of pancreatic beta-cell mass andfunction in db/db mice

删除 Elovl6 可防止 db/db 小鼠胰腺 β 细胞质量和功能的衰竭

• 发表时间: 2012
• 作者: Yuta Nakano;Takashi Matsuzaka;NieTang;Marii Suzuki;Noriko Suzuki-Kemuriyama;Motoko Kuba;KeitaShoshi;Tomotaka Yokoo;Yoshimi Nakagawa,Kiyoaki Ishii;Masako Shimada;HitoshiIwasaki;Kazuto Kobayashi;Shigeru Yatoh,Akimitsu Takahashi;Naoya Yahagi;Hi
• 通讯作者: Hi

Dicer has a crucial role in the early stage of adipocyte differentiation, but not in lipid synthesis, in 3T3-L1 cells

• DOI: 10.1016/j.bbrc.2012.03.110
• 发表时间: 2012-04-20
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Fujimoto, Yuri;Nakagawa, Yoshimi;Shimano, Hitoshi
• 通讯作者: Shimano, Hitoshi

TFE3regulates muscle metabolic geneexpression

TFE3调节肌肉代谢基因表达

• 发表时间: 2012
• 期刊: increases glycogen stores,and enhances insulin sensitivity in mice
• 作者: Iwasaki H;Naka A;Iida KT;NakagawaY;Matsuzaka T;Ishii KA;Kobayashi K,Takahashi A;Yatoh S;Yahagi N;Sone H,Suzuki H;Yamada N;Shimano H.
• 通讯作者: Shimano H.

Elovl6 promotes nonalcoholic steatohepatitis in mice and humans.

Elovl6 会促进小鼠和人类的非酒精性脂肪性肝炎。

• 作者: 松坂 賢;齋藤 亮;唐澤 直義;松森 理恵;煙山 紀子;中川 嘉;石井 清朗;岩崎 仁;小林 和人;矢藤 繁;高橋 昭光;曽根 博仁;鈴木 浩明;矢作 直也;山田 信博;島野 仁;島野 仁;島野 仁;Yuta Nakano;Takashi Matsuzaka
• 通讯作者: Takashi Matsuzaka

筑波大学内分泌代謝・糖尿病内科

筑波大学内分泌代谢与糖尿病系