Development of the biomarker for suicide prediction using the methylation at the gene promoters

利用基因启动子甲基化开发自杀预测生物标志物

• 批准号: 23659566
• 负责人: MORINOBU Shigeru
• 金额: $ 2.25万
• 依托单位: Kochi University (2013)Hiroshima University (2011-2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659566/
• 关键词: DNAメチル化; うつ病; 自殺予測; バイオマーカー; BDNF; SLC6A4; 不遇な養育環境; 自殺; SLC3A4; 5-HT受容体; NTRK2; 5-HTT; メチル化

To prevent the suicide in major depression, we measured the methylation levels at the promoter of exon I of the brain-derived neurotrophic factor, Trk B receptor, serotonin transporter (5HTT), and serotonin2c receptor gene in 50 patients with major depression and examined the possibility of the epigenetic biomarker for suicide prediction. We extracted DNA from peripheral blood and measured the methylation levels at the 149 CpGs in these 4 genes using MassARRAY system. No significant relationship between the methylation level of each CpG and the severity of suicidal idea evaluated by the Hamilton Rating Scale for Depression was found. However, there were significant correlations betweeen the methylation levels of 5HTT CpGs and the sevirity of eraly adversities evaluated by the Early TRauma Inventry. Based on these findings, it is postulated taht the methylation levels of CpGs in the 5HTT gene may be an appropriate biomarker for suicide prediction.

为了预防重度抑郁症患者自杀，我们检测了50例重度抑郁症患者脑源性神经营养因子、Trk B受体、5 -羟色胺转运蛋白（5HTT）和5 -羟色胺2c受体基因外显子I启动子的甲基化水平，并研究了这种表观遗传生物标志物预测自杀的可能性。我们从外周血中提取DNA，使用MassARRAY系统测量这4个基因中149个CpGs的甲基化水平。各CpG甲基化水平与汉密尔顿抑郁评定量表评定的自杀意念严重程度无显著相关。然而，5HTT CpGs的甲基化水平与早期创伤量表评估的早期逆境严重程度之间存在显著相关性。基于这些发现，我们推测5HTT基因中CpGs的甲基化水平可能是预测自杀的一个合适的生物标志物。

项目成果

Possibility of DNA methyl ation profiles as a potent diagnostic biomarker in PTSD

DNA 甲基化谱作为 PTSD 有效诊断生物标志物的可能性

• 发表时间: 2013
• 作者: Segawa M;Morinobu S;Fuchikami M;Fujita Y;Okada S;Okamoto Y;Yamawaki S;Morinobu S
• 通讯作者: Morinobu S

Searching for epigenetic biomarkers in PTSD

寻找 PTSD 的表观遗传生物标志物

• 发表时间: 2014
• 作者: 岡田怜;淵上学;森信繁;Morinobu S
• 通讯作者: Morinobu S

Searching for epigenetic biomarkers in major depression : focusing on the sero tonin signal transduction

寻找重性抑郁症的表观遗传生物标志物：关注血清素信号转导

• 发表时间: 2013
• 作者: Segawa M;Morinobu S;Fuchikami M;Fujita Y;Okada S;Okamoto Y;Yamawaki S
• 通讯作者: Yamawaki S

気分障害の薬理・生化学

情绪障碍的药理学和生物化学

• 发表时间: 2012
• 作者: 森信繁;淵上学
• 通讯作者: 淵上学

DNA methylation profiles of the brain-derived neurotrophic factor (BDNF) gene as a potent diagnostic biomarker in psychiatric disorders.

脑源性神经营养因子 (BDNF) 基因的 DNA 甲基化谱作为精神疾病的有效诊断生物标志物。

• 发表时间: 2011
• 作者: Fukuyama;K.;Tanahashi;S.;Nakagawa;M.;Yamamura;S.;Motomura;E.;Shiroyama;T.;Tanii;H.;Okada;M;Okada S et al.
• 通讯作者: Okada S et al.