Establishment and application of in-vitro hypertrophic and degeneration model designed to clarify the mechanism of mechanical signal transduction in chondrocytes.

本研究旨在建立体外软骨细胞肥大退变模型，以阐明软骨细胞机械信号转导的机制。通过对不同静水压力条件下软骨细胞退变标志物表达的检测，结果表明，20 Mpa压力1小时是诱导小鼠成软骨ATDC5细胞和小鼠原代关节软骨细胞退变的最佳刺激条件。

The purpose of this study is to establish in-vitro hypertrophic and degeneration model designed to clarify the mechanism of mechanical signal transduction in chondrocytes. The examination of expressions of degeneration markers of chondrocytes under various conditions of hydrostatic pressure revealed that pressure of 20MPa for 1 hour was the optimum stimulus condition to induce degenerative change in mouse chondrogenic ATDC5 cells and mouse primary articular chondrocytes.