Development of experimental system to analyze the involvement of bone marrow during lung metastasis

骨肉瘤患者的预后取决于肺转移。近年来，骨髓在建立肿瘤转移中的作用逐渐为人们所认识。在本研究中，我们首次报道了间充质干细胞在成骨分化过程中细胞运动和运动方式变化的机制-骨肉瘤的起源。接下来，我们比较了我们实验室建立的高转移小鼠骨肉瘤细胞系LM8和亲代Dunn细胞之间的肺转移能力。LM8和DUNN在小鼠骨髓中均未发现，但在两种细胞系的外渗(侵袭肺内皮细胞)过程中存在显著差异。因此，我们报道了在小鼠骨肉瘤中建立肺转移的侵袭能力的损害。

Prognosis of patients suffering osteosarcoma depends on lung metastasis. Recently the role of bone marrow in the establishment of metastasis shed lights. In this study, we firstly reported the mechanism of change in the cellular motility and motile mode during the osteoblastic differentiation of mesenchymal stem cells-origin of osteosarcoma. Next, we compared the ability of lung metastasis between highly metastatic mouse osteosarcoma cell line, LM8-establisged in our laboratory, and parental Dunn cells. Neither LM8 nor Dunn found in the mouse bone marrow, while striking difference exist during the step of extravasation (invasion onto the endothelial cells of lung) between two cell lines. We thus reported the involvements of invasive ability to establish lung metastasis in mouse osteosarcoma.