functional reconstruction of axon with using a giant axon of squid in vivo

点击翻译按钮获取中文摘要

Nerve transplantation is applied clinically as a valid strategy for regeneration, but it may not be obtained an effect entirely satisfactory in many cases. We conceived to construct the experimental connecting of the axons before occurring Waller degeneration. For this purpose, a squid giant axon was used. For overcoming the nociceptive reaction of active squid beyond the scope of the assumption, it was established anesthesia methods for severe invasive study. I also have established a conduction velocity measurement method and axonal stimulation huge electrophysiological. It was difficult to establish live imaging to observe the recovery process of axonal transport using a fluorescent marker at the same time, because it needed high magnification of microscope for observing mitochondrial staining. Now we started to dye axonal flow by changing the target.