Minimal reprogramming of cultured MSC based on DNA methylation analysis

本研究的目的是建立安全可靠的再生医学技术，利用培养的间充质干细胞（MSCs），通过最小的重编程重置。在本研究中，我们广泛分析了培养大鼠间充质干细胞基因组的DNA甲基化，并确定了甲基化随细胞退化的变化。通过刺激DNA去甲基化，研究了一种改善DNA去甲基化状态的方法，并将该方法应用于猪骨再生模型。

The purpose of this research is to establish safe and reliable technology for regenerative medicine using the cultured mesenchymal stem cells (MSCs), which are reset by minimum reprogramming. In this research, we analyzed DNA methylation of cultured rat MSCs genome-widely, and identified the variations of methylation with cell deterioration. By using stimulus to DNA demethylation,we examined a method to improve the state of the methylation, and applied the method to a bone regeneration model in pig.