Development of artificial organelle through the controlled cellular uptake of polyion complex vesicles

到目前为止，我们已经开发出了大小控制良好的多离子复合物囊泡（PICsomes），其大小范围在100至400纳米之间。picsome的特点是具有生物相容性和半透性囊泡膜。因此，装载酶的PICsomes有望用于酶基纳米反应器，它可以在细胞内作为人工细胞器工作。在这个研究项目中，我们尝试利用picsome开发“人工细胞器”。通过将酶包封在PICsomes中而不使其失活，成功建立了纳米反应器的制备方法。此外，我们还研究了化学修饰的picsome与细胞之间的相互作用，并评估并发现了进一步细胞摄取的主要因素。结果表明，PIC膜的交联程度和picsome的可变形性在一定程度上影响了细胞的摄取。

We have so far developed polyion complex vesicles (PICsomes) with well-controlled size in the size ranging from 100 to 400 nm. PICsomes are characterized by their biocompatibility and semipermeable vesicle membrane. Therefore, enzyme-loaded PICsomes are promising for enzyme-based nanoreactors, which can work inside of cells as an artificial organelle. In this research project, we tried to develop "artificial organelle"utilizing PICsomes. The preparation method of nano-reactors was successfully established through encapsulation of enzymes in PICsomes without deactivation. Also, we investigated the interaction between chemically-modified PICsomes and cells, and evaluated and found major factors for further cellular uptake. Results indicated that degree of cross-linking of PIC membrane and deformability of PICsomes contribute cellular uptake to some extent.