Development of artificial organelle through the controlled cellular uptake of polyion complex vesicles

到目前为止，我们已经开发了聚离子复合物囊泡（PIC囊泡），其尺寸范围从100至400 nm。PICsome具有生物相容性和半透性囊泡膜的特点。因此，负载酶的PICsome有望用于基于酶的纳米反应器，其可以作为人工细胞器在细胞内工作。在本研究计划中，我们试图利用PIC体开发“人工细胞器”。成功地建立了一种将酶包埋在PIC微球中而不失活的纳米反应器的制备方法。此外，我们研究了化学修饰的PIC体和细胞之间的相互作用，并评估和发现进一步细胞摄取的主要因素。结果表明，PIC膜的交联程度和PIC体的变形能力在一定程度上有助于细胞摄取。

We have so far developed polyion complex vesicles (PICsomes) with well-controlled size in the size ranging from 100 to 400 nm. PICsomes are characterized by their biocompatibility and semipermeable vesicle membrane. Therefore, enzyme-loaded PICsomes are promising for enzyme-based nanoreactors, which can work inside of cells as an artificial organelle. In this research project, we tried to develop "artificial organelle"utilizing PICsomes. The preparation method of nano-reactors was successfully established through encapsulation of enzymes in PICsomes without deactivation. Also, we investigated the interaction between chemically-modified PICsomes and cells, and evaluated and found major factors for further cellular uptake. Results indicated that degree of cross-linking of PIC membrane and deformability of PICsomes contribute cellular uptake to some extent.