The regulation of bone metabolism by amino acid

氨基酸对骨代谢的调节

• 批准号: 23689004
• 负责人: HINOI Eiichi
• 金额: $ 17.47万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23689004/
• 关键词: 骨代謝; 骨芽細胞; 破骨細胞; 遺伝子欠損マウス; アミノ酸; 骨粗鬆症

Although amino acid transporters are indispensable for a variety of cellular events in eukaryotic cells, little attention has been paid to their functional role in bone modeling and remodeling so far. Here, we have attempted to demonstrate the possible involvement of a system A amino acid transporter Slc38a1 in the regulation of osteoblastogenesis and osteoclastogenesis in vivo. To that end, cell-specific (osteoblast and osteoclast) knockout mice were generated. In vertebrae of those knockout mice, no marked change was seen in bone volume over tissue volume, osteoblastic parameter and osteoclastic parameter compared with wild-type (WT) mice by bone histomorphometric analyses. Moreover, ovariectomy resulted in a marked reduction of vertebrae bone volume in both WT mice and osteoblast-specific Slc38a1 knockout mice.

虽然氨基酸转运蛋白在真核细胞的许多细胞事件中是不可或缺的，但到目前为止，很少有人关注它们在骨建模和重建中的功能作用。在这里，我们试图证明可能参与系统A的氨基酸转运蛋白Slc38a1在体内成骨细胞和破骨细胞的调节。为此，产生细胞特异性（成骨细胞和破骨细胞）敲除小鼠。在这些敲除小鼠的椎骨中，通过骨组织形态计量学分析，与野生型（WT）小鼠相比，骨体积相对于组织体积、成骨细胞参数和骨密度参数没有明显变化。此外，卵巢切除术导致WT小鼠和成骨细胞特异性Slc 38 a1敲除小鼠的椎骨骨体积显着减少。

项目成果

Negative correlation between Per1 and Sox6 expression during chondrogenic differentiation in pre-chondrocytic ATDC5 cells

前软骨细胞 ATDC5 细胞成软骨分化过程中 Per1 和 Sox6 表达呈负相关

• 发表时间: 2013
• 期刊: J. Pharmacol. Sci
• 作者: Le;N.Q.;Binh;N.T.;Takarada;T.;Takarada-Iemata;M.;Hinoi;E. and Yoneda;Y
• 通讯作者: Y

Positive Regulation of Osteoclastic Differentiation by Growth Differentiation Factor 15 Upregulated in Osteocytic Cells Under Hypoxia

• DOI: 10.1002/jbmr.1538
• 发表时间: 2012-04-01
• 期刊: JOURNAL OF BONE AND MINERAL RESEARCH
• 影响因子: 6.2
• 作者: Hinoi, Eiichi;Ochi, Hiroki;Yoneda, Yukio
• 通讯作者: Yoneda, Yukio

Negative correlation between Per1 and Sox6 expression during chondrogenic differentiation in pre-chondrocytic ATDC5 cells.

前软骨细胞 ATDC5 细胞软骨形成分化过程中 Per1 和 Sox6 表达呈负相关。

• 发表时间: 2013
• 期刊: J Pharmacol Sci.
• 作者: Le NQ;Binh NT;Takarada T;Takarada-Iemata M;Hinoi E;Yoneda Y.
• 通讯作者: Yoneda Y.

Growth differentiation factor-15を介した骨細胞 による破骨細胞活性化調節

骨细胞通过生长分化因子 15 调节破骨细胞活化

• 作者: 田坂元一;生沼泉;根岸学;檜井栄一
• 通讯作者: 檜井栄一

PossiblePossible involvement of glutamatergic signaling machineries in pathophysiology of rheumatoid arthritis

谷氨酸信号传导机制可能参与类风湿性关节炎的病理生理学

• 发表时间: 2011
• 期刊: J. Pharmacol. Sci
• 作者: Hinoi;E. and Yoneda;Y
• 通讯作者: Y