Development of new therapeutic tools against pathogenic bacteria

开发针对病原菌的新治疗工具

• 批准号: 23689027
• 负责人: KIM Minsoo
• 金额: $ 17.8万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23689027/
• 关键词: エフェクター; 病原細菌; 阻害剤; エフェクタ-

Many pathogenic bacteria, including Shigella, enteropathogenic Escherichia coli (EPEC), and enterohemorrhagic E.coli (EHEC), are associated with diarrheal diseases in young children in developing countries. Although these pathogens are a significant health threat in the developing world, many Japanese people have suffered from O157 infection. However, no effective vaccines have yet been developed. Multi-drug resistant bacteria are increasing problem. Therefore, we aim to develop new therapeutic tools to counteract the progression of these diseases.To develop therapeutic tools against Shigella infection, we have analyzed the role of OspE during Shigella infection. ospE family genes are highly conserved among many enteropathogenic bacteria, including Shigella, O157, Citrobacter rodentium, and Salmonella, suggesting that OspE plays a general role in bacterial infection. We show here that OspE homologs play a key role in the establishment of during other pathogenic bacteria infection.

许多病原菌，包括志贺氏菌、肠致病性大肠埃希菌(EPEC)和肠出血性大肠杆菌(EHEC)，与发展中国家幼儿腹泻疾病有关。虽然这些病原体在发展中国家是一个重大的健康威胁，但许多日本人都感染了O157。然而，目前还没有研制出有效的疫苗。多重耐药细菌的问题日益严重。因此，我们的目标是开发新的治疗工具来对抗这些疾病的进展。为了开发针对志贺氏菌感染的治疗工具，我们分析了OSPE在志贺氏菌感染中的作用。OSPE家族基因在志贺氏菌、O157、轮状柠檬酸杆菌和沙门氏菌等肠道致病菌中高度保守，提示OSPE在细菌感染中起普遍作用。我们在这里表明，OSPE同源物在其他病原菌感染期间的建立中发挥了关键作用。

项目成果

病原細菌感染における宿主翻訳後修飾

致病细菌感染的宿主翻译后修饰

• 发表时间: 2012
• 作者: 長田康孝;三條場千寿;木村純二;後藤康之;松本芳嗣;Yoshio Mori;矢尾育子;金玟秀
• 通讯作者: 金玟秀

病原性バクテリアによるユビキチン修飾系の制御

病原菌对泛素修饰系统的调控

• 发表时间: 2012
• 期刊: 医学のあゆみ
• 作者: Otsuki N;Abo H;Kubota T;Mori Y;Umino Y;Okamoto K;Takeda M;Komase K;矢尾育子;Kim Minsoo
• 通讯作者: Kim Minsoo

Real-time, lable-free monitoring of poly ubiquitin chain formation with bacterial ubiquitin ligase

使用细菌泛素连接酶实时、无标记地监测多聚泛素链的形成

• 发表时间: 2013
• 作者: 佐藤慶治;高屋明子;山本友子;伊東史子;木村旭;金玟秀
• 通讯作者: 金玟秀

Regulation of host cell-cycle progression by bacterial pathogens

细菌病原体对宿主细胞周期进程的调节

• 发表时间: 2011
• 作者: Yao I;Matsumura S;Katano T;Yamagata K;Imoto S;Ito S;松井優里;金玟秀
• 通讯作者: 金玟秀

Monoubiquitination of Tob/BTG family proteins competes with degradation-targeting polyubiquitination.

Tob/BTG 家族蛋白的单泛素化与靶向降解的多泛素化竞争。

• DOI: 10.1016/j.bbrc.2011.04.107
• 发表时间: 2011
• 期刊: Biochem Biophys Res Commun
• 作者: Fujishita T;Aoki M;and Taketo MM.;武部直明;井尻成保・高塚直・加藤絵里香・足立伸次;Suzuki T
• 通讯作者: Suzuki T