Establishment of periodontal tissue regeneration method by using of novel PDLSC cell surface marker and PDL marker

利用新型PDLSC细胞表面标记物和PDL标记物建立牙周组织再生方法

• 批准号: 23689077
• 负责人: WADA Naohisa
• 金额: $ 17.47万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23689077/
• 关键词: 歯根膜組織; 間葉系幹細胞; 再生; 歯根膜; 再生医療

We found that Semaphorin 3A (Sema3A) can induce mesenchymal-stem-like properies in human periodontal ligament (PDL) cells, and Wnt5a can induce collagen fibrillogenesis which is main component of PDL tissue, by human PDL cells (HPDLC). Sema3A-overexpressing HPDLC exhibited an enhanced capacity to differentiate into both functional osteoblasts and adipocytes, and upregulated the expression of stem cell markers. Meanwhile, Wnt5a suppressed osteoblastic differentiation of HPDLC, while it significantly enhanced proliferation and migration of HPDLC, and PDL-related gene expression and collagen production in HPDLC. Both knockdown of periostin gene expression and inhibition of TGF-beta1 function suppressed the Wnt5a-induced PDL-related gene expression and collagen production. Although further studies are required to clarify the precise functional mechanism of both factors, Sema3A and Wnt5a can be factors with potential to regenerate PDL tissue efficiently.

我们发现Semaphorin 3A（Sema 3A）能诱导人牙周膜细胞（HPDLC）向间充质干细胞样分化，Wnt 5a能诱导人牙周膜细胞（HPDLC）向胶原纤维样分化。Sema 3A过表达的HPDLC表现出增强的分化为功能性成骨细胞和脂肪细胞的能力，并上调干细胞标志物的表达。同时，Wnt 5a抑制HPDLC的成骨分化，促进HPDLC的增殖和迁移，促进HPDLC中PDL相关基因的表达和胶原的生成。骨膜蛋白基因表达的敲低和TGF-β 1功能的抑制抑制Wnt 5a诱导的PDL相关基因表达和胶原蛋白的产生。虽然需要进一步的研究来阐明这两种因子的确切功能机制，但Sema 3A和Wnt 5a可能是具有有效再生PDL组织潜力的因子。

项目成果

Conversion of periodontal ligament cells into multipotent stem-like cells

牙周膜细胞向多能干细胞的转化

• 发表时间: 2013
• 作者: Kono K;Maeda H;Fujii S;Tomokiyo A;Yamamoto N;Wada N;Monnouchi S;Teramatsu Y;Hamano S;Koori K;Akamine A;矢田純一;沢;Yamamoto et al;沢;Koori et al;Wada et al.
• 通讯作者: Wada et al.

再生医療における歯原性幹細胞の可能性

牙源干细胞在再生医学中的潜力

• 发表时间: 2013
• 作者: Mimura T;Usui T;Yamagami S;Funatsu H;Noma H;Toyono T;Mori M;Amano S;石沢 武彰;和田尚久
• 通讯作者: 和田尚久

Differentiation of Periodontal Ligament Stem /Progenitor Cells : Roles of TGF-β1. In : Stem Cells and Cancer Stem Cells, volume 4, Therapeutic Applications in Disease and Injury. Hayat MA (ed)(Chapter 5)

牙周膜干细胞/祖细胞的分化：TGF-β1 的作用：干细胞和癌症干细胞，第 4 卷，疾病和损伤的治疗应用（Hayat MA）（第 5 章）。

• 发表时间: 2012
• 作者: Maeda H;Fujii S;Monnouchi S;Wada N;Akamine A
• 通讯作者: Akamine A

Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells.

• DOI: 10.1089/scd.2013.0405
• 发表时间: 2014-09
• 期刊: Stem cells and development
• 影响因子: 4
• 作者: Naohisa Wada;H. Maeda;Daigaku Hasegawa;S. Gronthos;P. Bartold;D. Menicanin;S. Fujii;S. Yoshida;Atsushi Tomokiyo;Satoshi Monnouchi;A. Akamine

Wnt5aはTGFβ1を介してヒト歯根膜細胞のコラーゲン線維形成を促進する

Wnt5a通过TGFβ1促进人牙周膜细胞胶原纤维形成

• 发表时间: 2014
• 作者: 長谷川大学;和田尚久;前田英史;吉田晋一郎;門野内聡;御手洗裕美;濱野さゆり;祐田明香;赤峰昭文
• 通讯作者: 赤峰昭文