Fabrication of Complex Hydrogel Materials with Microscale Patterns for Cell Organization

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We developed microfluidic systems for preparing complex hydrogel materials embedding micrometer-patterns with different physicochemical properties. Complex hydrogel fibers, stripe-patterned sheets, cell-sized ECM particles, and planar patterns were fabricated. These materials were applied to, for example, the coculture of primary hepatocytes and non-parenchymal cells, the formation of heterogeneous spheroids incorporating ECM particles, and the preparation of relatively large tissues by combining these unit materials. In addition, strategies for preparing vascular tissue models have been proposed using microfluidic devices made of hydrogel.