Preparation of stimuli-responsive surfaces that release cationic polymer triggered by thiol-disulfide exchange for gene introduction

在本研究中，我们旨在通过制备一种新的刺激响应型多阳离子释放表面，以提高经济、低细胞毒性基因导入方法之一的反向转染的效率。用半胱氨酸作为刺激检测多阳离子释放。结果表明，在含有半胱氨酸的水溶液中，多阳离子从表面释放出来，对细胞无毒。此外，293T细胞与表面粘附良好，表面显示出细胞毒性活性。这一功能表面有可能成为基因导入的实验平台。

In this study, we aimed to improve the efficiency of reverse transfection, one of the economic and low cytotoxic gene introduction methods, by preparing novel stimuli-responsive polycation releasing surface. Polycation release was examined by using cysteine as stimuli. As a result, polycation was released from the surface by applying cysteine containing aqueous solution which was nontoxic to cells. Moreover, 293T cells were well adhered to the surface and surface showed any cytotoxic activities. This functional surface would be potentially useful as experimental platform for gene introduction.