Preparation of stimuli-responsive surfaces that release cationic polymer triggered by thiol-disulfide exchange for gene introduction
Preparation of stimuli-responsive surfaces that release cationic polymer triggered by thiol-disulfide exchange for gene introduction
制备刺激响应表面，通过硫醇-二硫化物交换触发释放阳离子聚合物以进行基因导入
批准号：
23700566
负责人：
AKIMOTO Aya
金额：
$2.75万
依托单位：
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2011
资助国家：
日本
项目状态：
已结题
起止时间：
2011 至 2012
中文摘要
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英文摘要
In this study, we aimed to improve the efficiency of reverse transfection, one of the economic and low cytotoxic gene introduction methods, by preparing novel stimuli-responsive polycation releasing surface. Polycation release was examined by using cysteine as stimuli. As a result, polycation was released from the surface by applying cysteine containing aqueous solution which was nontoxic to cells. Moreover, 293T cells were well adhered to the surface and surface showed any cytotoxic activities. This functional surface would be potentially useful as experimental platform for gene introduction.
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会议论文
Preparation of cell-culturing glass surfaces that release branched polyethyleneimine triggered by thiol–disulfide exchange
制备细胞培养玻璃表面，通过硫醇-二硫化物交换触发释放支化聚乙烯亚胺
制备细胞培养玻璃表面，通过硫醇-二硫化物交换触发释放支化聚乙烯亚胺
DOI：
10.1016/j.colsurfb.2012.10.031
发表时间：
2013
期刊：
Colloids and Surfaces B: Biointerfaces
影响因子：
--
作者：
[坂野 貴宣, 弓場 英司, 原田 敦史, 河野 健司, 石原俊也,鄭雄一,酒井崇匡, Aya Mizutani Akimoto]
通讯作者：
Aya Mizutani Akimoto