Isolation for an antibacterial substance produced by Vibrio vulnificus and its characterization

创伤弧菌产生的抗菌物质的分离及其表征

• 批准号: 23700881
• 负责人: YOKOCHI Nana
• 金额: $ 1.08万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23700881/
• 关键词: ビブリオ; バルニフィカス・抗菌物質; V. vulnificus; 抗菌物質; バクテリオシン; vibrio vulnificus

We isolated for antibacterial substance with an inhibitory activity against Vibrio vulnificus. V. vulnificus L-9 showed antibacterial activity against 45 V. vulnificus strains among 51 stock cultures maintained by our laboratory. The antibacterial substance produced by V. vulnificus L-9 inhibited several strains of Vibrio spices, gram negative bacteria such as Escherichia coli, gram positive bacteria such as Staphylococcus aureus，Bacillus subtilis, Lactobacillus reuteri and several strains of Lactococcus spices. The antibacterial substance was sensitive to Protease K, was stable in the pH 3-6, and wasin the high salt concentration (24%). The apparent molecular mass of antibacterial substance was estimated to be lower than 2,000 Da, by the ultrafiltration. These results indicated that the antibacterial substance produced by V. vulnificus L-9 was a novel bacteriocin with broad spectrum antibacterial activity.

我们分离出了对创伤弧菌具有抑制活性的抗菌物质。创伤弧菌L-9对我室保存的51株创伤弧菌中的45株具有抗菌活性。创伤弧菌L-9产生的抗菌物质对几株调味品弧菌、革兰氏阴性菌如大肠杆菌、革兰氏阳性菌如金黄色葡萄球菌、枯草芽孢杆菌、雷氏乳杆菌和几株乳球菌调味品都有抑制作用。该抗菌物质对K酶敏感，在pH 3~6范围内稳定，在高盐浓度(24%)下稳定。超滤法测得抗菌物质的表观分子质量小于2,000 Da。这些结果表明创伤弧菌L-9产生的抗菌物质是一种具有广谱抗菌活性的新型细菌素。

项目成果

Vibrio vulnificus の遺伝型解析

创伤弧菌基因型分析

• 发表时间: 2011
• 作者: 横地奈菜;田中重光;松本浩一;大石浩隆;中島幹夫;神田康三;小林元太
• 通讯作者: 小林元太

有明海由来乳酸菌が生産する抗菌性物質

有明海乳酸菌产生的抗菌物质

• 发表时间: 2012
• 作者: 横地奈菜;渡辺真理,黒田沙織,田中重光,麻生祐司,神田康三,小林元太;横地奈菜;平地藍他
• 通讯作者: 平地藍他

Vibrio vulnificus 由来抗菌物質の生産条件検討

创伤弧菌抗菌物质生产条件的研究

• 发表时间: 2011
• 作者: 横地奈菜;渡辺真理,黒田沙織,田中重光,麻生祐司,神田康三,小林元太;横地奈菜;平地藍他;鷹川恵利;横地奈菜,他;渡辺真理,他;渡辺真理,他
• 通讯作者: 渡辺真理,他

Vibrio vulnificus 由来抗菌物質の抗菌スペクトル

创伤弧菌抗菌物质的抗菌谱

• 发表时间: 2011
• 作者: 横地奈菜;他
• 通讯作者: 他

Distribution of virulence markers among Vibrio vulnificus isolates of clinical and environmental origin and regional characteristics in Japan.

• DOI: 10.1371/journal.pone.0055219
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Yokochi N;Tanaka S;Matsumoto K;Oishi H;Tashiro Y;Yoshikane Y;Nakashima M;Kanda K;Kobayashi G
• 通讯作者: Kobayashi G