Isolation for an antibacterial substance produced by Vibrio vulnificus and its characterization

我们分离了一种对创伤弧菌具有抑制活性的抗菌物质。创伤弧菌L-9对本实验室保存的51株创伤弧菌中的45株具有抗菌活性。创伤弧菌L-9产生的抗菌物质对多种香料弧菌、大肠杆菌等革兰氏阴性菌、金黄色葡萄球菌、枯草芽孢杆菌、罗伊氏乳杆菌等革兰氏阳性菌和多种香料乳球菌均有抑制作用。该抗菌物质对蛋白酶K敏感，在pH 3-6范围内稳定，在高盐浓度（24%）下稳定。超滤法测得抗菌物质的表观分子量小于2,000 Da。结果表明，创伤弧菌L-9产生的抗菌物质是一种新的具有广谱抗菌活性的细菌素。

We isolated for antibacterial substance with an inhibitory activity against Vibrio vulnificus. V. vulnificus L-9 showed antibacterial activity against 45 V. vulnificus strains among 51 stock cultures maintained by our laboratory. The antibacterial substance produced by V. vulnificus L-9 inhibited several strains of Vibrio spices, gram negative bacteria such as Escherichia coli, gram positive bacteria such as Staphylococcus aureus，Bacillus subtilis, Lactobacillus reuteri and several strains of Lactococcus spices. The antibacterial substance was sensitive to Protease K, was stable in the pH 3-6, and wasin the high salt concentration (24%). The apparent molecular mass of antibacterial substance was estimated to be lower than 2,000 Da, by the ultrafiltration. These results indicated that the antibacterial substance produced by V. vulnificus L-9 was a novel bacteriocin with broad spectrum antibacterial activity.