Distribution of virulence markers among Vibrio vulnificus isolates of clinical and environmental origin and regional characteristics in Japan.
Distribution of virulence markers among Vibrio vulnificus isolates of clinical and environmental origin and regional characteristics in Japan.
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DOI：
10.1371/journal.pone.0055219
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Kobayashi G
中科院分区：
文献类型：
--
作者：
Yokochi N;Tanaka S;Matsumoto K;Oishi H;Tashiro Y;Yoshikane Y;Nakashima M;Kanda K;Kobayashi G
创伤弧菌是一种机会致病菌，广泛分布于河口环境，可引起坏死性筋膜炎和败血症。在日本，根据流行病学研究，创伤弧菌的发病率集中在九州，主要是在有明海的沿海地区。为了检查毒力潜力，最近开发了各种基因分型方法。本研究旨在调查沿海3个不同感染率地区临床和环境来源的创伤弧菌分离株毒力标记的分布情况，并确定这些分离株是否具有铁载体编码基因viuB。我们研究了16S核糖体核糖核酸（rRNA）基因，vvhA，vcg和荚膜多糖（CPS）的基因型分布，以及从日本患者和环境样本中收集的156株病毒中存在viuB。环境样品采集自三个沿海地区：有明海、伊势和三川湾以及加拉津湾。结果表明，基因型的比例取决于样品的来源的差异。将从患者获得的创伤弧菌分离株分类为所有基因型的临床类型。在环境分离株中，16S rRNA基因、vvhA和vcg基因型的临床型比例为有明海>伊势&三川湾>加拉津湾。CPS分析显示无显著性差异。大多数分离物具有viuB。有明海中存在大量临床型创伤弧菌。三个不同感染率的沿海地区显示出不同的基因型比例。这可能表明临床分离株的分布与创伤弧菌感染的发生率相关。
Vibrio vulnificus is an opportunistic human pathogen that is widely distributed in estuarine environments and is capable of causing necrotizing fasciitis and sepsis. In Japan, based on epidemiological research, the incidences of V. vulnificus were concentrated in Kyusyu, mainly in coastal areas of the Ariake Sea. To examine the virulence potential, various genotyping methods have recently been developed. This study aimed to investigate the distribution of virulence markers among V. vulnificus isolates of clinical and environmental origin in three coastal areas with different infection incidences and to determine whether these isolates have the siderophore encoding gene viuB. We examined the distribution of genotypes of the 16S ribosomal ribonucleic acid (rRNA) gene, vvhA, vcg, and capsular polysaccharide (CPS), and the presence of viuB in 156 isolates collected from patients and environmental samples in Japan. The environmental samples were collected from three coastal areas: the Ariake Sea, Ise & Mikawa Bay, and Karatsu Bay. The results showed disparity in the ratios of genotypes depending on the sample origins. V. vulnificus isolates obtained from patients were classified into the clinical type for all genotypes. In the environmental isolates, the ratios of the clinical type for genotypes of the 16S rRNA gene, vvhA, and vcg were in the order of the Ariake Sea>Ise & Mikawa Bay>Karatsu Bay. Meanwhile, CPS analysis showed no significant difference. Most isolates possessed viuB. Many V. vulnificus belonging to the clinical type existed in the Ariake Sea. Three coastal areas with different infection incidences showed distinct ratios of genotypes. This may indicate that the distribution of clinical isolates correlates with the incidence of V. vulnificus infection.
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影响因子：
4.4
作者：
Biosca, EG;Amaro, C;Pedersen, K
通讯作者：
Pedersen, K
影响因子：
2.2
作者：
Matsumoto, Kouichi;Ohshige, Kenji;Oishi, Hirotaka
通讯作者：
Oishi, Hirotaka
影响因子：
2.8
作者：
Panicker, G;Vickery, MCL;Bej, AK
通讯作者：
Bej, AK
DOI：
10.1016/0190-9622(91)70059-b
发表时间：
1991-03-01
影响因子：
13.8
作者：
PARK, SD;SHON, HS;JOH, NJ
通讯作者：
JOH, NJ
影响因子：
168.9
作者：
Bisharat, N;Agmon, V;Farmer, JJ
通讯作者：
Farmer, JJ