Evaluation of a simple and quick method of vitamin B_<12> quantitation by the instrumental analysis

仪器分析评价一种简单快速的维生素B_<12>定量方法

• 批准号: 23700946
• 负责人: MATSUMOTO Teruki
• 金额: $ 2.75万
• 依托单位: National Institute of Health and Nutrition
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23700946/
• 关键词: 栄養学; 食品; 分析化学; ビタミン; 定量分析; ビタミンB12; LC/MS

A microbiological assay which is cumbersome and less precise was used widely for quantitation of vitamin B_<12>, but we examined a new method using the high performance liquid chromatograph (HPLC) as a simple, easy and quick analysis method. It was suggested that condensation of analyte and removal of impurities by using solid phase extraction cartridge was allowed the quantitation of ultralow concentration of vitamin B_<12> in HPLC

维生素B_的微生物测定法<12>是一种简便、快速的测定方法。结果表明，采用固相萃取柱对待测物进行浓缩和除杂，可用于超低浓度维生素B_（1-x）的高效液相色谱定量<12>