Restoration of extinct plant population using the seed of herbarium specimens and development of specimen management method

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I investigated the enzyme activity of the embryo and germination test of herbarium specimens's seed of endangered plant of 555 specimens of 117 species. As a result, the seeds of 235 specimens (42%) of 73 species (62%) were alive. The oldest survival specimen seed was Saussurea pulchella (Fisch. ex Hornem.) Fisch. that has been collected 87 years ago.I alos examined the effect of specimen preparation method has on the viability of the seed. Specimen seeds subjected to drying treatment at a temperature lower than 40 degrres C showed a high survival rate.And I compared the genetic diversity of seedling populations of specimens of Vincetoxicum pycnostelma Kitag. and the wild populations from which the specimens were collected. Genetic diversity of the seedlings were significantly lower than that of wild populations, but the alleles which were lost from the wild population were confirmed.