Analysis of the mechanism for liver cancer developing from non-alcoholic steatohepatitis by next-generation sequencing

二代测序分析非酒精性脂肪性肝炎发展为肝癌的机制

• 批准号: 23701082
• 负责人: KITAMOTO Takuya
• 金额: $ 2.83万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23701082/
• 关键词: ゲノムワイド相関解析; 非アルコール性脂肪肝炎; 次世代シークエンサー; PNPLA3; SAMM50; PARVB; 次世代シークエンス; 異所性脂肪蓄積

For GWAS, 372 Japanese NAFLD subjects and 934 control individuals were analyzed. For replication studies, 172 NAFLD and 1012 control subjects were monitored. Rs738409 in the PNPLA3 gene was most strongly associated with NAFLD. Rs2896019, and rs381062 in the PNPLA3 gene, rs738491, rs3761472, and rs2143571 in the SAMM50 gene, rs6006473, rs5764455, and rs6006611 in the PARVB gene had also significant and were associated with decreased serum triglycerides and increased AST and ALT in NAFLD patients. These SNPs were associated with steatosis grade and NAS. Rs738409, rs2896019, rs738491, rs6006473, rs5764455, and rs6006611 were associated with fibrosis. We amplified approximately 150kb region including PNPLA3 of 19 NAFLD and 9 control subjects and analyzed by next-generation sequencing.

对于GWAS，分析了372名日本NAFLD受试者和934名对照个体。在重复研究中，对172名NAFLD患者和1012名对照患者进行了监测。PNPLA3基因中的Rs738409与NAFLD的相关性最强。PNPLA3基因中的Rs2896019、rs381062， SAMM50基因中的rs738491、rs3761472、rs2143571， PARVB基因中的rs6006473、rss5764455、rs6006611也与NAFLD患者血清甘油三酯降低、AST和ALT升高相关。这些snp与脂肪变性等级和NAS相关。Rs738409、rs2896019、rs738491、rs6006473、rss5764455和rs6006611与纤维化相关。我们扩增了19名NAFLD和9名对照受试者的约150kb区域，包括PNPLA3，并通过下一代测序进行分析。

项目成果

薬物代謝関連遺伝子群の高密度、高精度な遺伝子型決定法の確立

药物代谢相关基因群高密度、高精度基因分型方法的建立

• 发表时间: 2011
• 作者: 北本綾;北本卓也;水澤精穂;寺西啓;堀田紀久子;上嶋健治;小杉眞司;中尾一和;関根章博
• 通讯作者: 関根章博

次世代シーケンサーを用いた薬物代謝関連遺伝子群のターゲットリシーケンス

使用下一代测序仪对药物代谢相关基因进行靶向重测序

• 发表时间: 2012
• 作者: Takemori N;Takemori A;Matsuoka K;Matsushita N;Takeda H;Sawasaki T;Endo Y;Higashiyama S;北本綾
• 通讯作者: 北本綾

Replication Study of 15 Recently Published Loci for Body Fat Distribution in the Japanese Population

• DOI: 10.5551/jat.14589
• 发表时间: 2013-01-01
• 期刊: JOURNAL OF ATHEROSCLEROSIS AND THROMBOSIS
• 影响因子: 4.4
• 作者: Hotta, Kikuko;Kitamoto, Aya;Sekine, Akihiro
• 通讯作者: Sekine, Akihiro

Genetic variations in the CYP17A1 and NT5C2 genes are associated with a reduction in visceral and subcutaneous fat areas in Japanese women

• DOI: 10.1038/jhg.2011.127
• 发表时间: 2012-01-01
• 期刊: JOURNAL OF HUMAN GENETICS
• 影响因子: 3.5
• 作者: Hotta, Kikuko;Kitamoto, Aya;Sekine, Akihiro
• 通讯作者: Sekine, Akihiro

PNPLA3遺伝子上のrs738409と非アルコール性脂肪肝炎とのケース・コントロール相関解析

PNPLA3基因rs738409与非酒精性脂肪性肝炎的病例对照相关性分析

• 发表时间: 2011
• 作者: 堀田紀久子;北本卓也;北本綾;米田正人;中島淳;上野登隆;兵庫秀幸;越智秀典;茶山一彰;関根章博;中尾一和
• 通讯作者: 中尾一和