Metaproteomics of viral lysates in a marine Synechococcus

海洋聚球藻病毒裂解物的宏蛋白质组学

• 批准号: 23710011
• 负责人: YOSHIDA Mitsuhiro
• 金额: $ 2.91万
• 依托单位: Japan Agency for Marine-Earth Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23710011/
• 关键词: 物質循環; 溶存有機物; プロテオミクス; 微生物ループ; 海洋; シアノバクテリア; ファージ

In this study, the comprehensive metaproteomic analysis using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was performed to investigate the dissolved (< 0.2 um) proteomic composition of viral lysates occurring during infection of a marine cyanobacterial Synechococcus sp. The analysis of the lysate proteome revealed that photosynthetic proteins, metabolic enzymes involved in amino acids and carbohydrates, and viral capsids were major components, possibly indicating the formation of dissolved proteins during viral infection was influenced by differences in the nature and subcellular localization of protein molecules.

本研究采用液相色谱-串联质谱法对蛋白质组进行了全面的元蛋白质组学分析进行LC-MS/MS方法以研究溶解的（< 0.2 μ m）的蛋白质组组成。裂解物蛋白质组的分析表明，光合蛋白，涉及氨基酸和碳水化合物的代谢酶，病毒衣壳是主要成分，这可能表明病毒感染过程中溶解蛋白的形成受到蛋白质分子性质和亚细胞定位差异的影响。

项目成果

Metagenomic analyses of viral communities in (hado-)pelagic sediments

(hado-)远洋沉积物中病毒群落的宏基因组分析

• 发表时间: 2011
• 作者: Mitsuhiro Yoshida;Takuro Nunoura;Yoshihiro Takaki;and Ken Takai
• 通讯作者: and Ken Takai

Effect of sucrose and halophilic lactic acid bacterium Tetragenococcus halophilus on chemical characteristics and microbial proliferation during fish sauce fermentation

蔗糖和嗜盐乳酸菌嗜盐四联球菌对鱼酱发酵过程中化学特性和微生物增殖的影响

• DOI: 10.1111/j.1745-4565.2012.00392.x
• 发表时间: 2012
• 期刊: J. Food Safety
• 作者: Shozen K-I;Satomi M;Yano Y;Yoshida M;Fukui Y;Takano T;Funatsu Y
• 通讯作者: Funatsu Y

Bacterial communities in fish sauce mash using culture-dependent and -independent methods

使用依赖于培养和独立于培养的方法研究鱼酱糊中的细菌群落

• DOI: 10.2323/jgam.58.273
• 发表时间: 2012
• 期刊: The Journal of General and Applied Microbiology
• 作者: Fukui Y;Yoshida M;Shozen K-I;Funatsu Y;Takano T;Oikawa H;Yano Y;Satomi M
• 通讯作者: Satomi M

Metagenomic analyses of the viral communities in (hado)pelagic sediments ((超)深海における表層堆積物中の未知ウイルス群集構造の解明)

(hado) 远洋沉积物中病毒群落的宏基因组分析

• 发表时间: 2012
• 作者: 吉田光宏*;高木善弘;布浦拓郎;高井研
• 通讯作者: 高井研