Risk assessment of methyl mercury toxicity using fibroblast

使用成纤维细胞进行甲基汞毒性风险评估

• 批准号: 23710044
• 负责人: HORAI Sawako
• 金额: $ 2.83万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23710044/
• 关键词: マングース; 初代肝細胞; 水銀; 細胞毒性; 水銀蓄積; 線維芽細胞; リスク評価; 野生動物

The present study established a primary hepatocyte culture for the small Indian mongoose (Herpestes auropunctatus). To determine the suitable medium for growing the primary hepatic cells of this species, we compared the condition of cells cultured in three media that are frequently used for mammalian cell culture: Dulbecco's Modified Eagle’s Medium (DMEM), RPMI-1640, and William’s E. Of these, William’s E medium was best suited for culturing the hepatic cells of this species. Using periodic acid-Schiff (PAS) staining and ultrastructural observations, we demonstrated the cells collected from mongoose livers were hepatocytes. To evaluate the distribution of mercury(Hg) in the liver tissue, we carried out autometallography (AMG) staining. Most of the Hg compounds were found in the central region of hepatic lobules. Smooth endoplasmic reticulum, which plays a role in xenobiotic metabolism, lipid/cholesterol metabolism, and the digestion and detoxification of lipophilic substances is grown in this area. This suggested that Hg colocalized with smooth endoplasmic reticulum. The results of the present study could be useful to identify the detoxification systems of wildlife with high Hg content in the body, and to evaluate the susceptibility of wildlife to Hg toxicity.

本研究建立了小印度猫鼬（斑疱疹猫鼬）的原代肝细胞培养。为了确定适合该物种肝细胞原代培养的培养基，我们比较了三种常用的哺乳动物细胞培养培养基：Dulbecco's Modified Eagle 's medium （DMEM）、rmi -1640和William 's E培养基中细胞的培养条件。其中，William 's E培养基最适合该物种肝细胞的培养。通过周期性酸-希夫（PAS）染色和超微结构观察，我们证实从猫鼬肝脏中收集的细胞是肝细胞。为了评估汞（Hg）在肝组织中的分布，我们进行了自金相（AMG）染色。汞化合物主要分布在肝小叶的中心区域。光滑内质网在这一区域生长，它在外源代谢、脂质/胆固醇代谢以及亲脂物质的消化和解毒中起作用。这表明汞与光滑内质网共定位。本研究结果可用于鉴定体内高汞含量野生动物的解毒系统，以及评价野生动物对汞毒性的敏感性。

项目成果

Arsenic and Mn levels in Isaza (Gymnogobius isaza) during the mass mortality event in Lake Biwa, Jspan

日本琵琶湖大规模死亡事件期间 Isaza (Gymnogobius isaza) 的砷和锰含量

• DOI: 10.1016/j.envpol.2011.05.007
• 发表时间: 2011
• 期刊: Environmental Pollution
• 影响因子: 8.9
• 作者: Sawako Horai Hirata;Daisuke Hayase;Akifumi Eguchi;Takaaki Itai;Kei Nomiyama;Tomohiko Isobe;Tetsuro Agusa;Toshiyuki Ishikawa;Michio Kumagai;Shinsuke Tanabe
• 通讯作者: Shinsuke Tanabe