Investigation of tetrodotoxin biosynthesis based on the genome sequence analysis of the potential producer microorganisms

基于潜在生产微生物基因组序列分析的河豚毒素生物合成研究

• 批准号: 23710252
• 负责人: KUDO Fumitaka
• 金额: $ 3万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23710252/
• 关键词: 生合成; 微生物ゲノム解析; テトロドトキシン; 微生物ゲノム解読; Shewanella algae; Vibrio alginolyticus; テトロドトキシン生産性; 生合成遺伝子; Pseudoalteromonas; ゲノムマイニング; 特異生合成遺伝子

The genome sequence analysis of Shewanella algae JCM 21037, Pseudoalteromonas tetraodonis JCM 21038, and Vibrio alginolyticus NBRC 15630 that are deposited as tetrodotoxin (TTX) and/or its homolog producers were performed to identify the TTX biosynthetic gene clusters. Although three hundreds of characteristic genes were found in Vibrio alginolyticus by comparison with same genus of bacteria, any obvious gene cluster that seems to be responsible for the TTX production was not identified. Therefore, it was speculated that the TTX biosynthetic genes might spread out in the genomic DNA. Otherwise, they are maybe smaller number of genes than that of initial expectation. This might suggest that TTX could be biosynthesized from relatively large size of molecules rather than small primary metabolites. Thus, in future, such advanced analogs of TTX wouldbe utilized as potential substrates to identify TTX biosynthetic enzymes.

Shewanella algae JCM 21037，假胞虫蛋白酶TETRAODONIS JCM 21038的基因组序列分析以及作为四毒素（TTX）沉积的弧菌NBRC 15630和/或其同源性生产者的基因组序列分析。尽管通过与细菌属进行比较，在弧菌藻溶液中发现了三百个特征基因，但似乎尚未鉴定出任何造成TTX产生的明显基因簇。因此，据推测，TTX生物合成基因可能分布在基因组DNA中。否则，它们的基因数量可能少于最初的预期。这可能表明可以从相对较大的分子而不是小原代代谢物中生物合成TTX。因此，将来，TTX的这种高级类似物将用作潜在底物来鉴定TTX生物合成酶。