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ガーリックオイルの主要成分と生体防御系機能との関連

大蒜油主要成分与生物防御系统功能的关系

基本信息

批准号：
24500971
负责人：
立屋敷かおる
金额：
$ 3.41万
依托单位：
Joetsu University of Education
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2012
资助国家：
日本
起止时间：
2012-04-01 至 2014-03-31
项目状态：
已结题

项目摘要

Diallyl disulfide (DADS) is a major sulfur compound of garlic. However, it still remains unclear how DADS affects the distribution of white blood cell subsets. We studied the dose-dependent effects of DADS administration on the circulating number of white blood cells (WBCs) and lymphocyte subsets in rats. Next we studied the effects of DADS on the number of lymphocytes subsets in the spleen. The spleen was harvested 4 hour after administration, and then the splenic cells were isolated, and the total number of cells was counted. Male 10-week-old SD rats were divided into the DADS-free and DADS-orally administered (dose=10, 20, and 40mg/kg BW) groups. Blood samples were collected from the tail vein at 0, 1, 2, 4 and 6 hour after the administration. DADS administration reduced dose- and time-dependently the circulating number of total WBCs, T-lymphocytes, B-lymphocytes and monocytes. DADS administration increased dose- and time-dependently plasma corticosterone concentration. DADS administration increased acutely the number of splenic cells, especially B-lymphocytes. These results demonstrated that acutely the distribution of lymphocyte subsets and monocytes between the peripheral blood and spleen along with the increase of plasma corticosterone. In particular, DADS administration reduced the number of B-lymphocytes in the blood while increased that in the spleen, suggesting that circulating B-lymphocytes were migrated into the spleen in response to DADS administration at least in part via the action of corticosterone.

二烯丙基二硫（DADS）是大蒜中的主要含硫化合物。然而，目前仍不清楚DADS如何影响白色血细胞亚群的分布。我们研究了DADS给药对大鼠循环白色血细胞（WBC）和淋巴细胞亚群的剂量依赖性影响。接下来，我们研究了DADS对脾脏中淋巴细胞亚群数量的影响。给药后4小时收获脾脏，然后分离脾细胞，并计数细胞总数。将10周龄雄性SD大鼠分为DADS组和DADS组（剂量分别为10、20和40 mg/kg BW）。在给药后0、1、2、4和6小时从尾静脉采集血样。DADS给药可剂量依赖性和时间依赖性地减少总WBC、T淋巴细胞、B淋巴细胞和单核细胞的循环数量。DADS给药增加血浆皮质酮浓度的剂量和时间依赖性。DADS给药急性增加脾细胞，特别是B淋巴细胞的数量。结果表明，急性期外周血淋巴细胞亚群和单核细胞在脾脏和外周血中的分布随血浆皮质酮水平的升高而沿着变化。特别是，DADS给药减少了血液中B淋巴细胞的数量，同时增加了脾脏中的B淋巴细胞的数量，这表明循环B淋巴细胞至少部分地通过皮质酮的作用迁移到脾脏中。