Anti-fungal garlic-derived polysulfides:- Modes of action and applications in food crop protection (HAMILTON_U17ICASE)

由于许多化学农药对环境和健康的影响，现在迫切需要在粮食作物保护计划中替代害虫控制方法。大蒜提取物对许多粮食作物害虫具有广谱活性，提供了有吸引力的害虫控制替代品，这些替代品是可生物降解的，对人类和动物福利的风险较小。大蒜提取物中最突出的生物活性成分是一系列不寻常的有机硫分子，称为二烯丙基多硫化物（DAPS）。初步研究表明，一旦DAPS进入目标病原体，它们的生物活性可以通过与巯基辅因子如谷胱甘肽反应来触发，以扰乱细胞内氧化还原状态并最终导致细胞死亡。该项目与ECOspray（http：//www.ecospray.com/）合作，旨在：1.从生化水平揭示DAPS如何诱导真菌病原体的氧化应激.揭示这是如何与他们的影响真菌生命周期的不同阶段3相关。在田间试验环境中，使用这些信息来优化DAPS治疗方案，以保护许多粮食作物（例如：马铃薯、豌豆、番茄）防治真菌害虫。

Prompted by environmental and health concerns associated with many chemical pesticides there is now an urgent need for alternative pest control methods in food crop protection programs.Garlic extracts display broad spectrum activity against numerous food crop pests, offering appealing pest control alternatives, which are biodegradable and pose less risk to humans and animal welfare. The most prominent bioactive components in garlic extracts are a series of unusual organosulfur molecules known as diallyl-polysulfides (DAPS). Preliminary studies indicate that once DAPS enter the target pathogen their bioactivity can be triggered by reaction with thiol cofactors such as glutathione to perturb the intracellular redox status and ultimately cause cell death. In collaboration with ECOspray (http://www.ecospray.com/) this project aims to:1. Unravel how DAPS induce oxidative stress in fungal pathogens at the biochemical level.2. Reveal how this correlates with their effects different stages of the fungal life cycle3. Use this information, in field trial settings, to optimise DAPS treatment regimens to protect numerous food crops (eg. Potato, pea, tomato) against fungal pests.