Anti-fungal garlic-derived polysulfides:- Modes of action and applications in food crop protection (HAMILTON_U17ICASE)

由于与许多化学农药有关的环境和健康问题，现在迫切需要在粮食作物保护计划中寻找其他害虫控制方法。大蒜提取物对许多粮食作物害虫具有广谱活性，提供了有吸引力的害虫防治替代品，这些替代品是可生物降解的，对人类和动物福利的风险较小。大蒜提取物中最显著的生物活性成分是一系列罕见的有机硫分子，称为二烯丙基多硫化物（DAPS）。初步研究表明，一旦DAPS进入目标病原体，其生物活性可通过与硫醇辅助因子（如谷胱甘肽）的反应而被触发，从而扰乱细胞内氧化还原状态，最终导致细胞死亡。与ECOspray （http://www.ecospray.com/）合作，该项目旨在：揭示DAPS如何在生化水平上诱导真菌病原体氧化应激2。揭示这与真菌生命周期不同阶段的影响之间的关系。利用这些信息，在田间试验环境中，优化DAPS处理方案，以保护众多粮食作物（如玉米）。马铃薯，豌豆，番茄)抗真菌害虫。

Prompted by environmental and health concerns associated with many chemical pesticides there is now an urgent need for alternative pest control methods in food crop protection programs.Garlic extracts display broad spectrum activity against numerous food crop pests, offering appealing pest control alternatives, which are biodegradable and pose less risk to humans and animal welfare. The most prominent bioactive components in garlic extracts are a series of unusual organosulfur molecules known as diallyl-polysulfides (DAPS). Preliminary studies indicate that once DAPS enter the target pathogen their bioactivity can be triggered by reaction with thiol cofactors such as glutathione to perturb the intracellular redox status and ultimately cause cell death. In collaboration with ECOspray (http://www.ecospray.com/) this project aims to:1. Unravel how DAPS induce oxidative stress in fungal pathogens at the biochemical level.2. Reveal how this correlates with their effects different stages of the fungal life cycle3. Use this information, in field trial settings, to optimise DAPS treatment regimens to protect numerous food crops (eg. Potato, pea, tomato) against fungal pests.