Study on the molecular mechanism controlled by SPACIA1, which associated with synoviocyte proliferation.

SPACIA1控制与滑膜细胞增殖相关的分子机制研究。

• 批准号: 24791009
• 负责人: KOMATSU RIE
• 金额: $ 2.66万
• 依托单位: St. Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791009/
• 关键词: 滑膜炎; 細胞増殖; SPACIA1

SPACIA1 is a novel gene associated with abnormal synovial proliferation which is one of the major characteristics of rheumatoid arthritis (RA). In this study, we aimed to a precise understanding of the molecular mechanisms behind this process. We analyzed constituents of purified SPACIA1 protein complex using shotgun proteomics. We identified CDK6, one of functional genes at G1 phase, which gene expression is regulated by SPACIA1 in mRNA turnover. CDK6 knockdown by its siRNA inhibited the proliferation of cultured RA synovial fibroblasts. These results suggested that CDK6 is functionally involved in RA synoviocyte proliferation. Specific inhibition of CDK6 may be a useful therapeutic strategy for RA.

SPACIA1是一个与滑膜异常增殖相关的新基因，滑膜异常增殖是类风湿关节炎(RA)的主要特征之一。在这项研究中，我们的目的是精确地了解这一过程背后的分子机制。我们用鸟枪式蛋白质组学方法分析了纯化的SPACIA1蛋白复合体的组成。我们发现CDK6是一个处于G1期的功能基因，该基因的表达受SPACIA1调节。CDK6被其siRNA抑制培养的RA滑膜成纤维细胞的增殖。提示CDK6在功能上参与了RA滑膜细胞的增殖。特异性抑制CDK6可能是治疗类风湿关节炎的有效策略。

项目成果

Analysis of a Core Promoter Context of Synoviocyte Proliferation-Associated in Collagen Induced Arthritis 1 (SPACIA1) Gene Associated with Synovitis.

与滑膜炎相关的胶原诱导关节炎 1 (SPACIA1) 基因中滑膜细胞增殖相关的核心启动子背景分析。

• 发表时间: 2012
• 期刊: J St. Marianna Univ.
• 作者: Fujii R;Komatsu R;Sato T;Yagishita N;Aratani S;Araya N;Nishikawa H;Yamano Y;Yudoh K,Beppu M;Nishioka K;Nakajima T;Fujii Ryoji
• 通讯作者: Fujii Ryoji

Analysis of a Core Promoter Context of Synoviocyte Proliferation- Associated in Collagen Induced Arthritis 1 (SPACIA1) Gene Associated with Synovitis.

与滑膜炎相关的胶原诱导关节炎 1 (SPACIA1) 基因中滑膜细胞增殖相关的核心启动子背景分析。

• 发表时间: 2012
• 期刊: J St. Marianna Univ.
• 作者: Fujii R;Komatsu R;Sato T;Yagishita N;Aratani S;Araya N;Nishikawa H;Yamano Y;Yudoh K,Beppu M;Nishioka K;Nakajima T
• 通讯作者: Nakajima T

CDK6 transcripts expression control by the synoviocyte proliferation gene, SPACIA1.

CDK6 转录本的表达受滑膜细胞增殖基因 SPACIA1 控制。

• 作者: Rie Komatsu;Ryoji Fujii;Tomoo Sato;Yoshihisa Yamano;Kazuo Yudoh;Moroe Beppu;Kusuki Nishioka;Toshihiro Nakajima
• 通讯作者: Toshihiro Nakajima

聖マリアンナ医科大学難病治療研究センター診断治療法開発部門

圣玛丽安娜大学医学院疑难杂症治疗研究中心诊断治疗开发部

聖マリアンナ医科大学 難病治療研究センター 診断治療法開発部門 業績一覧

圣玛丽安娜大学医学院疑难杂症治疗研究中心诊断治疗开发部成果一览