Experiments for fetal programming by embryonic ultrasound cardiography of mice

小鼠胚胎超声心动图胎儿编程实验

• 批准号: 24791114
• 负责人: AOYAGI Yoshimichi
• 金额: $ 1.5万
• 依托单位: Fukushima Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791114/
• 关键词: 胎児プログラミング; 血流再分配; 胎仔エコー

I gave mice in early pregnancy stress such as the drug load and examined an embryonic heart hemodynamic change by performing embryonic ultrasound cardiography. Heart rate , fractional shortening, and cardiac output tended to increase by subcutaneous injecting 0.2 mg/kg of nicotine to a pregnant mice 15-30 minutes after the injection. On the other hand in embryos, carotid artery flow significantly decreased after nicotine injection in comparison with the control group that injected normal saline , and dorsal aorta and umbilical artery flows tended to decrease 15-30 minutes after the injection.This circulation adjustment is a reaction to lead to the blood flow redistribution, and is considered to influence the fetal programming such as an embryonic growth retardation or postnatal developmental disorders.

我给小鼠在怀孕早期的压力，如药物负荷，并检查了胚胎心脏血流动力学的变化，通过执行胚胎超声心动图。 给妊娠小鼠皮下注射0.2 mg/kg尼古丁15-30分钟后，心率、短轴缩短率和心输出量趋于增加。另一方面，在胚胎中，与注射生理盐水的对照组相比，注射尼古丁后颈动脉流量显著减少，并且背主动脉和脐动脉流量在注射后15-30分钟趋于减少。这种循环调节是导致血流重新分布的反应，并且被认为影响胎儿编程，例如胚胎生长迟缓或出生后发育障碍。