Elucidation of the mechanism of blue-light reception by vibrational spectroscopy

通过振动光谱阐明蓝光接收机制

• 批准号: 5420312
• 负责人: Professor Dr. Joachim Heberle
• 金额: --
• 依托单位: Institut für Experimentalphysik
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5420312?language=en
• 关键词: Elucidation; mechanism; blue; light; reception

It is the aim of the project to achieve an atomic level of understanding of the mechanism of biological blue-light reception. Reaching this objective requires a methodology with acute sensitivity and sufficient time-resolution. These requirements are met by vibrational spectroscopy. The catalytic changes involve vibrational changes of the chromophore, the protein backbone and amino acid side chains. They can be as minute as alterations in hydrogen-bonding or protonation changes. The intermediates of the photoreaction along the reaction coordinate are either trapped by various techniques or directly traced in a time-resolved experiment. We will employ FT-IR spectroscopy which monitors virtually all vibrations of the protein. These data are compared to resonance-Raman spectroscopy which is selective for the vibrational modes of the chromophore. The biological systems under study are the LOV (Light Oxygen Voltage) domains, the BLUF (receptors of Blue Light Using FAD as chromophore) domains and the cryptochromes. Most of these samples will be provided by our collaborators. However, the cryptochrome CPH will be expressed in our lab. The project will greatly benefit from the structural studies of this collaborative effort on the one hand and from the expression of new blue-light photoreceptors on the other hand.

该项目的目标是实现对生物蓝光接收机制的原子水平的理解。实现这一目标需要一种具有敏锐灵敏度和足够的时间分辨率的方法。振动光谱学可以满足这些要求。催化变化包括发色团、蛋白质主链和氨基酸侧链的振动变化。它们可以像氢键或质子化的变化一样微小。沿着反应坐标的光反应中间体要么被各种技术捕获，要么在时间分辨实验中直接追踪。我们将使用傅里叶变换红外光谱来监测蛋白质的几乎所有振动。将这些数据与共振-拉曼光谱进行比较，这是对发色团的振动模式的选择。研究的生物系统包括光氧电压（LOV）结构域、蓝光受体（BLUF）结构域和隐色素。这些样品大部分将由我们的合作者提供。然而，隐色素CPH将在我们的实验室中表达。该项目将极大地受益于这种合作努力的结构研究，以及新的蓝光光感受器的表达。

项目成果

Solid-state photo-CIDNP effect observed in phototropin LOV1-C57S by (13)C magic-angle spinning NMR spectroscopy.

通过 (13)C 魔角旋转 NMR 光谱观察趋光素 LOV1-C57S 中的固态光 CIDNP 效应

• DOI: 10.1021/ja1082969
• 发表时间: 2010
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: S. S. Thamarath;J. Heberle;P. Hore;T. Kottke;J. Matysik
• 通讯作者: J. Matysik

Blue light induces global and localized conformational changes in the kinase domain of full-length phototropin.

• DOI: 10.1021/bi9016044
• 发表时间: 2010-02
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Anna Pfeifer;T. Mathes;Yinghong Lu;P. Hegemann;T. Kottke

Chromophore composition of a heterologously expressed BLUF-domain

异源表达的 BLUF 结构域的发色团组成

• DOI: 10.1039/b410923f
• 发表时间: 2004
• 期刊: Photochemical & Photobiological Sciences
• 影响因子: 3.1
• 作者: W. Laan;T. Bednarz;J. Heberle;K. J. Hellingwerf
• 通讯作者: K. J. Hellingwerf

Time-resolved Fourier transform infrared study on photoadduct formation and secondary structural changes within the phototropin LOV domain.

向光素 LOV 域内光加合物形成和二级结构变化的时间分辨傅里叶变换红外研究

• DOI: 10.1016/j.bpj.2008.11.016
• 发表时间: 2009
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: A. Pfeifer;T. Majerus;K. Zikihara;D. Matsuoka;S. Tokutomi;J. Heberle;T. Kottke
• 通讯作者: T. Kottke

Vibrational Spectroscopy Explores the Photoreaction of LOV Domains

振动光谱探索 LOV 域的光反应

• DOI: 10.1007/4-431-27092-2_17
• 发表时间: 2005
• 作者: J. Heberle