Prevalence of toxic Staphylococcus aureus and levels of antibody against toxin.

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To investigate the relationship between the levels of serum antibody to staphylococcal toxic shock antigen and effect of treatment, We planned to research the prevalence of toxic shock syndrome toxin 1 (TSST-1)-producing stains of staphylococcus aureus and antibody to TSST-1 among patients with minor trauma, burns, and skin bacterial infection. Before initiation of the study, we are required to obtain approval of Ethics Committee. I re-prepared the study plan and noticed the problems with statistical analysis. I keenly realize the importance of the designing clinical research and learned the epidemiological approach to improve the quality of study.